Identification of the major proteins of an immune modulating fraction from adult Fasciola hepatica released by Nonidet P40

R. M. Morphew; Clare M. Hamilton; H. A. Wright; David J. Dowling; Sandra M. O'Neill; P. M. Brophy

doi:10.1016/j.vetpar.2012.08.029

Identification of the major proteins of an immune modulating fraction from adult Fasciola hepatica released by Nonidet P40

R. M. Morphew, Clare M. Hamilton, H. A. Wright, David J. Dowling, Sandra M. O'Neill, P. M. Brophy

Adran y Gwyddorau Bywyd

Allbwn ymchwil: Cyfraniad at gyfnodolyn › Erthygl › adolygiad gan gymheiriaid

25 Dyfyniadau (Scopus)

70 Wedi eu Llwytho i Lawr (Pure)

Crynodeb

Fasciola hepatica NP-40 released protein extract (FhNPE) exhibits potent Th1 immunosuppressive properties in vitro and in vivo. However, the protein composition of this active fraction, responsible for Th1 immune modulatory activity, has yet to be resolved. Therefore, FhNPE, a Nonidet P-40 extract, was subjected to a proteomic analysis in order to identify individual protein components. This was performed using an in house F. hepatica EST database following 2D electrophoresis combined with de novo sequencing based mass spectrometry. The identified proteins, a mixture of excretory/secretory and membrane-associated proteins, are associated with stress response and chaperoning, energy metabolism and cytoskeletal components. The immune modulatory properties of these identified protein(s) are discussed and HSP70 from F. hepatica is highlighted as a potential host immune modulator for future study.

Iaith wreiddiol	Saesneg
Tudalennau (o-i)	379–385
Nifer y tudalennau	7
Cyfnodolyn	Veterinary Parasitology
Cyfrol	191
Rhif cyhoeddi	3-4
Dyddiad ar-lein cynnar	11 Medi 2012
Dynodwyr Gwrthrych Digidol (DOIs)	https://doi.org/10.1016/j.vetpar.2012.08.029
Statws	Cyhoeddwyd - 31 Ion 2013

Mynediad at Ddogfen

10.1016/j.vetpar.2012.08.029

120712 Morphew et alLlawysgrif awdur wedi’i dderbyn, 153 KB
Supplementary dataData, 617 KBTrwydded: CC BY

Cysylltiad parhaol

Dolen barhaus

Ffeiliau eraill a chysylltiadau

1 Wedi Gorffen

Developing a 'Validation Portfolio' to Exploit Key Virulence Proteins in Fasciola Species for Parasite Control
Brophy, P. (Prif Ymchwilydd)
Biotechnology and Biological Sciences Research Council
10 Meh 2010 → 09 Meh 2013
Prosiect: Ymchwil a ariannwyd yn allanol

Dyfynnu hyn

@article{a1232ea9b06e401a80cc95db7e8e6f05,

title = "Identification of the major proteins of an immune modulating fraction from adult Fasciola hepatica released by Nonidet P40",

abstract = "Fasciola hepatica NP-40 released protein extract (FhNPE) exhibits potent Th1 immunosuppressive properties in vitro and in vivo. However, the protein composition of this active fraction, responsible for Th1 immune modulatory activity, has yet to be resolved. Therefore, FhNPE, a Nonidet P-40 extract, was subjected to a proteomic analysis in order to identify individual protein components. This was performed using an in house F. hepatica EST database following 2D electrophoresis combined with de novo sequencing based mass spectrometry. The identified proteins, a mixture of excretory/secretory and membrane-associated proteins, are associated with stress response and chaperoning, energy metabolism and cytoskeletal components. The immune modulatory properties of these identified protein(s) are discussed and HSP70 from F. hepatica is highlighted as a potential host immune modulator for future study.",

keywords = "Anti-inflammatory, Heat shock protein, Proteomics, Amino Acid Sequence, Molecular Sequence Data, Electrophoresis, Gel, Two-Dimensional, Fasciola hepatica/chemistry, Immunologic Factors/chemistry, Sequence Alignment, Animals, Mass Spectrometry, Polyethylene Glycols/chemistry, Octoxynol, Helminth Proteins/chemistry",

author = "Morphew, {R. M.} and Hamilton, {Clare M.} and Wright, {H. A.} and Dowling, {David J.} and O'Neill, {Sandra M.} and Brophy, {P. M.}",

note = "Funding Information: The authors would like to thank Mr. Jim Heald and Dr. Charly Potter for their MS skills. The authors acknowledge the support of the BBSRC UK ( BBH0092561 ) and the Wellcome Trust (Grant WT094907MA ).",

year = "2013",

month = jan,

day = "31",

doi = "10.1016/j.vetpar.2012.08.029",

language = "English",

volume = "191",

pages = "379–385",

journal = "Veterinary Parasitology",

issn = "0304-4017",

publisher = "Elsevier",

number = "3-4",

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AU - Morphew, R. M.

AU - Hamilton, Clare M.

AU - Wright, H. A.

AU - Dowling, David J.

AU - O'Neill, Sandra M.

AU - Brophy, P. M.

N1 - Funding Information: The authors would like to thank Mr. Jim Heald and Dr. Charly Potter for their MS skills. The authors acknowledge the support of the BBSRC UK ( BBH0092561 ) and the Wellcome Trust (Grant WT094907MA ).

PY - 2013/1/31

Y1 - 2013/1/31

N2 - Fasciola hepatica NP-40 released protein extract (FhNPE) exhibits potent Th1 immunosuppressive properties in vitro and in vivo. However, the protein composition of this active fraction, responsible for Th1 immune modulatory activity, has yet to be resolved. Therefore, FhNPE, a Nonidet P-40 extract, was subjected to a proteomic analysis in order to identify individual protein components. This was performed using an in house F. hepatica EST database following 2D electrophoresis combined with de novo sequencing based mass spectrometry. The identified proteins, a mixture of excretory/secretory and membrane-associated proteins, are associated with stress response and chaperoning, energy metabolism and cytoskeletal components. The immune modulatory properties of these identified protein(s) are discussed and HSP70 from F. hepatica is highlighted as a potential host immune modulator for future study.

AB - Fasciola hepatica NP-40 released protein extract (FhNPE) exhibits potent Th1 immunosuppressive properties in vitro and in vivo. However, the protein composition of this active fraction, responsible for Th1 immune modulatory activity, has yet to be resolved. Therefore, FhNPE, a Nonidet P-40 extract, was subjected to a proteomic analysis in order to identify individual protein components. This was performed using an in house F. hepatica EST database following 2D electrophoresis combined with de novo sequencing based mass spectrometry. The identified proteins, a mixture of excretory/secretory and membrane-associated proteins, are associated with stress response and chaperoning, energy metabolism and cytoskeletal components. The immune modulatory properties of these identified protein(s) are discussed and HSP70 from F. hepatica is highlighted as a potential host immune modulator for future study.

KW - Anti-inflammatory

KW - Heat shock protein

KW - Proteomics

KW - Amino Acid Sequence

KW - Molecular Sequence Data

KW - Electrophoresis, Gel, Two-Dimensional

KW - Fasciola hepatica/chemistry

KW - Immunologic Factors/chemistry

KW - Sequence Alignment

KW - Animals

KW - Mass Spectrometry

KW - Polyethylene Glycols/chemistry

KW - Octoxynol

KW - Helminth Proteins/chemistry

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M3 - Article

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VL - 191

SP - 379

EP - 385

JO - Veterinary Parasitology

JF - Veterinary Parasitology

IS - 3-4

ER -

Identification of the major proteins of an immune modulating fraction from adult Fasciola hepatica released by Nonidet P40

Crynodeb

Mynediad at Ddogfen

Cysylltiad parhaol

Ffeiliau eraill a chysylltiadau

Ôl bys

Prosiectau

Developing a 'Validation Portfolio' to Exploit Key Virulence Proteins in Fasciola Species for Parasite Control

Dyfynnu hyn