Improved serodetection of Mycobacterium bovis infection in badgers (Meles meles) using multiantigen test formats

Rena Greenwald, Javan Esfandiari, Sandrine Lesellier, Raymond Houghton, John Pollock, Claus Aagaard, Peter Andersen, R. Glyn Hewinson, Mark Chambers*, Konstantin Lyashchenko

*Awdur cyfatebol y gwaith hwn

Allbwn ymchwil: Cyfraniad at gyfnodolynErthygladolygiad gan gymheiriaid


Despite attempts to control bovine tuberculosis, the incidence of disease in Great Britain continues to rise. In GB, the European badger (Meles meles) is a reservoir of infection with Mycobacterium bovis. In an effort to improve the serodetection of badger tuberculosis, we examined sera from M. bovis culture-positive and culture-negative badgers for their ability to recognize M. bovis antigens, using a multi-antigen print immunoassay (MAPIA). Depending on the antigens used in the MAPIA, the assay had a sensitivity of 49-59% and a specificity of 84-88% Results from the MAPIA were used to select antigens for the development of a lateral-flow immunoassay. This so-called 'Rapid Test' used 5μl of serum and gave unambiguous results within 10 min. When applied to 178 badger sera, the Rapid Test had a sensitivity of 53% and a specificity of 95%. This represented an improvement over the performance of the existing ELISA Test, which had a sensitivity of 47% and a specificity of 89% on the same sera. This is the first report of a diagnostic test for badger tuberculosis that can be performed alongside the captive animal.

Iaith wreiddiolSaesneg
Tudalennau (o-i)197-203
Nifer y tudalennau7
CyfnodolynDiagnostic Microbiology and Infectious Disease
Rhif cyhoeddi3
Dyddiad ar-lein cynnar08 Ebr 2003
Dynodwyr Gwrthrych Digidol (DOIs)
StatwsCyhoeddwyd - 01 Gorff 2003

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