Inactivation of the Mycobacterium bovis homologue of the polymorphic RD1 gene Rv3879c (Mb3909c) does not affect virulence

Jacqueline Inwald; Keith Jahans; R. Glyn Hewinson; Stephen V. Gordon

doi:10.1016/j.tube.2003.08.018

Inactivation of the Mycobacterium bovis homologue of the polymorphic RD1 gene Rv3879c (Mb3909c) does not affect virulence

Jacqueline Inwald, Keith Jahans, R. Glyn Hewinson, Stephen V. Gordon^*

^*Awdur cyfatebol y gwaith hwn

TB buchol, clefydau a rheolaeth

Animal Health and Veterinary Laboratories Agency

Allbwn ymchwil: Cyfraniad at gyfnodolyn › Erthygl › adolygiad gan gymheiriaid

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The RD1 locus is deleted from all strains of Mycobacterium bovis BCG but present in virulent isolates of M. bovis and Mycobacterium tuberculosis. The RD1 gene Rv3879c encodes a proline- and alanine-rich protein that shows sequence polymorphism across members of the M. tuberculosis complex. The role of this protein in virulence was investigated by deleting the Rv3879c homologue from M. bovis (Mb3909c) and testing the virulence of the mutant in the guinea pig model. The M. bovis ΔMb3909c mutant was not attenuated in the guinea pig model, showing that this gene does not encode a virulence factor and plays no role in the attenuation caused by loss of RD1. Crown

Iaith wreiddiol	Saesneg
Tudalennau (o-i)	387-393
Nifer y tudalennau	7
Cyfnodolyn	Tuberculosis
Cyfrol	83
Rhif cyhoeddi	6
Dynodwyr Gwrthrych Digidol (DOIs)	https://doi.org/10.1016/j.tube.2003.08.018
Statws	Cyhoeddwyd - 13 Tach 2003

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10.1016/j.tube.2003.08.018

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Ffeiliau eraill a chysylltiadau

Link to publication in Scopus

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@article{1078eb9eda4f4044b0addcba14f30be0,

title = "Inactivation of the Mycobacterium bovis homologue of the polymorphic RD1 gene Rv3879c (Mb3909c) does not affect virulence",

abstract = "The RD1 locus is deleted from all strains of Mycobacterium bovis BCG but present in virulent isolates of M. bovis and Mycobacterium tuberculosis. The RD1 gene Rv3879c encodes a proline- and alanine-rich protein that shows sequence polymorphism across members of the M. tuberculosis complex. The role of this protein in virulence was investigated by deleting the Rv3879c homologue from M. bovis (Mb3909c) and testing the virulence of the mutant in the guinea pig model. The M. bovis ΔMb3909c mutant was not attenuated in the guinea pig model, showing that this gene does not encode a virulence factor and plays no role in the attenuation caused by loss of RD1. Crown",

keywords = "Comparative genomics, Mycobacterium bovis, Virulence",

author = "Jacqueline Inwald and Keith Jahans and Hewinson, {R. Glyn} and Gordon, {Stephen V.}",

year = "2003",

month = nov,

day = "13",

doi = "10.1016/j.tube.2003.08.018",

language = "English",

volume = "83",

pages = "387--393",

journal = "Tuberculosis",

issn = "1472-9792",

publisher = "Elsevier",

number = "6",

}

TY - JOUR

T1 - Inactivation of the Mycobacterium bovis homologue of the polymorphic RD1 gene Rv3879c (Mb3909c) does not affect virulence

AU - Inwald, Jacqueline

AU - Jahans, Keith

AU - Hewinson, R. Glyn

AU - Gordon, Stephen V.

PY - 2003/11/13

Y1 - 2003/11/13

N2 - The RD1 locus is deleted from all strains of Mycobacterium bovis BCG but present in virulent isolates of M. bovis and Mycobacterium tuberculosis. The RD1 gene Rv3879c encodes a proline- and alanine-rich protein that shows sequence polymorphism across members of the M. tuberculosis complex. The role of this protein in virulence was investigated by deleting the Rv3879c homologue from M. bovis (Mb3909c) and testing the virulence of the mutant in the guinea pig model. The M. bovis ΔMb3909c mutant was not attenuated in the guinea pig model, showing that this gene does not encode a virulence factor and plays no role in the attenuation caused by loss of RD1. Crown

AB - The RD1 locus is deleted from all strains of Mycobacterium bovis BCG but present in virulent isolates of M. bovis and Mycobacterium tuberculosis. The RD1 gene Rv3879c encodes a proline- and alanine-rich protein that shows sequence polymorphism across members of the M. tuberculosis complex. The role of this protein in virulence was investigated by deleting the Rv3879c homologue from M. bovis (Mb3909c) and testing the virulence of the mutant in the guinea pig model. The M. bovis ΔMb3909c mutant was not attenuated in the guinea pig model, showing that this gene does not encode a virulence factor and plays no role in the attenuation caused by loss of RD1. Crown

KW - Comparative genomics

KW - Mycobacterium bovis

KW - Virulence

UR - http://www.scopus.com/inward/record.url?scp=0344873211&partnerID=8YFLogxK

U2 - 10.1016/j.tube.2003.08.018

DO - 10.1016/j.tube.2003.08.018

M3 - Article

C2 - 14623170

AN - SCOPUS:0344873211

SN - 1472-9792

VL - 83

SP - 387

EP - 393

JO - Tuberculosis

JF - Tuberculosis

IS - 6

ER -

Inactivation of the Mycobacterium bovis homologue of the polymorphic RD1 gene Rv3879c (Mb3909c) does not affect virulence

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