Induction of cellular immunity to a mycobacterial antigen adsorbed on lamellar particles of lactide polymers

N. Venkataprasad; A. G.A. Coombes; M. Singh; M. Rohde; K. Wilkinson; F. Hudecz; S. S. Davis; H. M. Vordermeier

doi:10.1016/S0264-410X(98)00372-7

Induction of cellular immunity to a mycobacterial antigen adsorbed on lamellar particles of lactide polymers

N. Venkataprasad, A. G.A. Coombes^*, M. Singh, M. Rohde, K. Wilkinson, F. Hudecz, S. S. Davis, H. M. Vordermeier

^*Awdur cyfatebol y gwaith hwn

TB buchol, clefydau a rheolaeth

Allbwn ymchwil: Cyfraniad at gyfnodolyn › Erthygl › adolygiad gan gymheiriaid

56 Dyfyniadau (Scopus)

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Microspheres prepared from synthetic, biodegradable poly (L-lactide) [PLA] and copolymers of lactide and glycolide such as poly (DL lactide co- glycolide) [PLG] have been widely investigated for controlled delivery of encapsulated vaccine antigens. In this study we describe novel lamellar microparticles produced from PLA to which protein antigens can be adsorbed. These particles when administered to mice, induced strong Th1-type T cell responses to the adsorbed 38 kDa protein antigen from M. tuberculosis characterised by high levels of Interferon-gamma. In addition to proteins, we were also able to adsorb synthetic peptides resulting in specific T cell proliferation. Induction of strong cellular immunity together with the versatility of antigen adsorption to these particles should make such lamellae a useful tool to deliver protective antigens from intracellular pathogens.

Iaith wreiddiol	Saesneg
Tudalennau (o-i)	1814-1819
Nifer y tudalennau	6
Cyfnodolyn	Vaccine
Cyfrol	17
Rhif cyhoeddi	15-16
Dyddiad ar-lein cynnar	18 Maw 1999
Dynodwyr Gwrthrych Digidol (DOIs)	https://doi.org/10.1016/S0264-410X(98)00372-7
Statws	Cyhoeddwyd - 09 Ebr 1999

NDC y CU

Mae’r allbwn hwn yn cyfrannu at y Nod(au) Datblygu Cynaliadwy canlynol

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10.1016/S0264-410X(98)00372-7

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Link to publication in Scopus

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@article{c184f7296a6445bebd799a98897e68c0,

title = "Induction of cellular immunity to a mycobacterial antigen adsorbed on lamellar particles of lactide polymers",

abstract = "Microspheres prepared from synthetic, biodegradable poly (L-lactide) [PLA] and copolymers of lactide and glycolide such as poly (DL lactide co- glycolide) [PLG] have been widely investigated for controlled delivery of encapsulated vaccine antigens. In this study we describe novel lamellar microparticles produced from PLA to which protein antigens can be adsorbed. These particles when administered to mice, induced strong Th1-type T cell responses to the adsorbed 38 kDa protein antigen from M. tuberculosis characterised by high levels of Interferon-gamma. In addition to proteins, we were also able to adsorb synthetic peptides resulting in specific T cell proliferation. Induction of strong cellular immunity together with the versatility of antigen adsorption to these particles should make such lamellae a useful tool to deliver protective antigens from intracellular pathogens.",

keywords = "Delivery system, Lactide polymers, Resorbable lamellae, Tuberculosis, Polyesters/chemistry, Drug Carriers, Lipoproteins/chemistry, Microspheres, Antigens, Bacterial/chemistry, Interleukin-4/analysis, Female, Peptide Fragments/chemical synthesis, Polyglactin 910/chemistry, Immunization, Mycobacterium tuberculosis/immunology, Lymphocyte Activation, Mice, Inbred C57BL, Cells, Cultured, Crystallization, Interferon-gamma/analysis, Animals, Adsorption, DNA/biosynthesis, Mice, Th1 Cells/immunology",

author = "N. Venkataprasad and Coombes, {A. G.A.} and M. Singh and M. Rohde and K. Wilkinson and F. Hudecz and Davis, {S. S.} and Vordermeier, {H. M.}",

year = "1999",

month = apr,

day = "9",

doi = "10.1016/S0264-410X(98)00372-7",

language = "English",

volume = "17",

pages = "1814--1819",

journal = "Vaccine",

issn = "0264-410X",

publisher = "Elsevier",

number = "15-16",

}

TY - JOUR

T1 - Induction of cellular immunity to a mycobacterial antigen adsorbed on lamellar particles of lactide polymers

AU - Venkataprasad, N.

AU - Coombes, A. G.A.

AU - Singh, M.

AU - Rohde, M.

AU - Wilkinson, K.

AU - Hudecz, F.

AU - Davis, S. S.

AU - Vordermeier, H. M.

PY - 1999/4/9

Y1 - 1999/4/9

N2 - Microspheres prepared from synthetic, biodegradable poly (L-lactide) [PLA] and copolymers of lactide and glycolide such as poly (DL lactide co- glycolide) [PLG] have been widely investigated for controlled delivery of encapsulated vaccine antigens. In this study we describe novel lamellar microparticles produced from PLA to which protein antigens can be adsorbed. These particles when administered to mice, induced strong Th1-type T cell responses to the adsorbed 38 kDa protein antigen from M. tuberculosis characterised by high levels of Interferon-gamma. In addition to proteins, we were also able to adsorb synthetic peptides resulting in specific T cell proliferation. Induction of strong cellular immunity together with the versatility of antigen adsorption to these particles should make such lamellae a useful tool to deliver protective antigens from intracellular pathogens.

AB - Microspheres prepared from synthetic, biodegradable poly (L-lactide) [PLA] and copolymers of lactide and glycolide such as poly (DL lactide co- glycolide) [PLG] have been widely investigated for controlled delivery of encapsulated vaccine antigens. In this study we describe novel lamellar microparticles produced from PLA to which protein antigens can be adsorbed. These particles when administered to mice, induced strong Th1-type T cell responses to the adsorbed 38 kDa protein antigen from M. tuberculosis characterised by high levels of Interferon-gamma. In addition to proteins, we were also able to adsorb synthetic peptides resulting in specific T cell proliferation. Induction of strong cellular immunity together with the versatility of antigen adsorption to these particles should make such lamellae a useful tool to deliver protective antigens from intracellular pathogens.

KW - Delivery system

KW - Lactide polymers

KW - Resorbable lamellae

KW - Tuberculosis

KW - Polyesters/chemistry

KW - Drug Carriers

KW - Lipoproteins/chemistry

KW - Microspheres

KW - Antigens, Bacterial/chemistry

KW - Interleukin-4/analysis

KW - Female

KW - Peptide Fragments/chemical synthesis

KW - Polyglactin 910/chemistry

KW - Immunization

KW - Mycobacterium tuberculosis/immunology

KW - Lymphocyte Activation

KW - Mice, Inbred C57BL

KW - Cells, Cultured

KW - Crystallization

KW - Interferon-gamma/analysis

KW - Animals

KW - Adsorption

KW - DNA/biosynthesis

KW - Mice

KW - Th1 Cells/immunology

UR - http://www.scopus.com/inward/record.url?scp=0033537879&partnerID=8YFLogxK

U2 - 10.1016/S0264-410X(98)00372-7

DO - 10.1016/S0264-410X(98)00372-7

M3 - Article

C2 - 10217578

AN - SCOPUS:0033537879

SN - 0264-410X

VL - 17

SP - 1814

EP - 1819

JO - Vaccine

JF - Vaccine

IS - 15-16

ER -

Induction of cellular immunity to a mycobacterial antigen adsorbed on lamellar particles of lactide polymers

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