Inferring cellular forces from image stacks

Jim H. Veldhuis, Ahmad Ehsandar, Jean Léon Maître, Takashi Hiiragi, Simon Cox, G. Wayne Brodland*

*Awdur cyfatebol y gwaith hwn

Allbwn ymchwil: Cyfraniad at gyfnodolynErthygladolygiad gan gymheiriaid

26 Dyfyniadau(SciVal)
320 Wedi eu Llwytho i Lawr (Pure)

Crynodeb

Although the importance of cellular forces to a wide range of embryogenesis and disease processes is widely recognized, measuring these forces is challenging, especially in three dimensions. Here, we introduce CellFIT-3D, a force inference technique that allows tension maps for three-dimensional cellular systems to be estimated from image stacks. Like its predecessors, video force microscopy and CellFIT, this cell mechanics technique assumes boundary-specific interfacial tensions to be the primary drivers, and it constructs force-balance equations based on triple junction (TJ) dihedral angles. The technique involves image processing, segmenting of cells, grouping of cell outlines, calculation of dihedral planes, averaging along three-dimensional TJs, and matrix equation assembly and solution. The equations tend to be strongly overdetermined, allowing indistinct TJs to be ignored and solution error estimates to be determined. Application to clean and noisy synthetic data generated using Surface Evolver gave tension errors of 1.6–7%, and analyses of eight-cell murine embryos gave estimated errors smaller than the 10% uncertainty of companion aspiration experiments. Other possible areas of application include morphogenesis, cancer metastasis and tissue engineering.

Iaith wreiddiolSaesneg
Rhif yr erthygl20160261
CyfnodolynPhilosophical Transactions B: Biological Sciences
Cyfrol372
Rhif cyhoeddi1720
Dynodwyr Gwrthrych Digidol (DOIs)
StatwsCyhoeddwyd - 19 Mai 2017

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