TY - JOUR
T1 - Inter-laboratory variation of in vitro cumulative gas production profiles of feeds using manual and automated methods
AU - Rymer, C.
AU - Williams, B. A.
AU - Brooks, Alison E.
AU - Davies, David R.
AU - Givens, D Ian
N1 - Rymer, C., Williams, B. A., Brooks, A. E., Davies, D. R., Givens, D. I. (2005). Inter-laboratory variation of in vitro cumulative gas production profiles of feeds using manual and automated methods. Animal Feed Science and Technology, 123-124, 225-241
PY - 2005/9/22
Y1 - 2005/9/22
N2 - A study was conducted to estimate variation among laboratories and between manual and automated techniques of measuring pressure on the resulting gas production profiles (GPP). Eight feeds (molassed sugarbeet feed, grass silage, maize silage, soyabean hulls, maize gluten feed, whole crop wheat silage, wheat, glucose) were milled to pass a 1 mm screen and sent to three laboratories (ADAS Nutritional Sciences Research Unit, UK; Institute of Grassland and Environmental Research (IGER), UK; Wageningen University, The Netherlands). Each laboratory measured GPP over 144 h using standardised procedures with manual pressure transducers (MPT) and automated pressure systems (APS). The APS at ADAS used a pressure transducer and bottles in a shaking water bath, while the APS at Wageningen and IGER used a pressure sensor and bottles held in a stationary rack. Apparent dry matter degradability (ADDM) was estimated at the end of the incubation. GPP were fitted to a modified Michaelis–Menten model assuming a single phase of gas production, and GPP were described in terms of the asymptotic volume of gas produced (A), the time to half A (B), the time of maximum gas production rate (tRM gas) and maximum gas production rate (RM gas). There were effects (P
AB - A study was conducted to estimate variation among laboratories and between manual and automated techniques of measuring pressure on the resulting gas production profiles (GPP). Eight feeds (molassed sugarbeet feed, grass silage, maize silage, soyabean hulls, maize gluten feed, whole crop wheat silage, wheat, glucose) were milled to pass a 1 mm screen and sent to three laboratories (ADAS Nutritional Sciences Research Unit, UK; Institute of Grassland and Environmental Research (IGER), UK; Wageningen University, The Netherlands). Each laboratory measured GPP over 144 h using standardised procedures with manual pressure transducers (MPT) and automated pressure systems (APS). The APS at ADAS used a pressure transducer and bottles in a shaking water bath, while the APS at Wageningen and IGER used a pressure sensor and bottles held in a stationary rack. Apparent dry matter degradability (ADDM) was estimated at the end of the incubation. GPP were fitted to a modified Michaelis–Menten model assuming a single phase of gas production, and GPP were described in terms of the asymptotic volume of gas produced (A), the time to half A (B), the time of maximum gas production rate (tRM gas) and maximum gas production rate (RM gas). There were effects (P
KW - Gas production
KW - Ring test
KW - Feed characterisation
KW - Manual pressure transducer
KW - Automated pressure system
U2 - 10.1016/j.anifeedsci.2005.04.029
DO - 10.1016/j.anifeedsci.2005.04.029
M3 - Article
SN - 0377-8401
VL - 123-124
SP - 123
EP - 124
JO - Animal Feed Science and Technology
JF - Animal Feed Science and Technology
IS - 1
ER -