Isolation of a gene expressed during sucrose accumulation in leaves of Lolium temulentum L.

A. L. Winters; J. Gallagher; C. J. Pollock; J. F. Parrar

doi:10.1093/jxb/46.special_issue.1345

Isolation of a gene expressed during sucrose accumulation in leaves of Lolium temulentum L.

A. L. Winters, J. Gallagher, C. J. Pollock, J. F. Parrar

Adran y Gwyddorau Bywyd

Allbwn ymchwil: Cyfraniad at gyfnodolyn › Erthygl › adolygiad gan gymheiriaid

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A 1620 base pair cDNA clone showing increased expression during sucrose accumulation in leaves of Lolium temulentum has been isolated by differential screening. Comparison of the DNA sequence of the open reading frame with published sequences has revealed extensive homology with sequences coding for ATP: L-methionme S-adenosylmethionine synthe-tase, EC 2.5.1.6 (SAM synthetase) (79.6% with Arabidopsis thaliana and 81.6% with Dianthus cary-ophyllus). Increased expression was observed in both intact seedling leaves and detached mature leaves during sucrose accumulation. Expression decreased during sucrose mobilization in leaves of intact seedlings. The significance of increased expression of a gene coding for SAM synthetase during sucrose accumulation is discussed in relation to the involvement of sucrose in the regulation of plant metabolism.

Iaith wreiddiol	Saesneg
Tudalennau (o-i)	1345-1350
Cyfnodolyn	Journal of Experimental Botany
Cyfrol	46
Rhif cyhoeddi	Special Issue
Dynodwyr Gwrthrych Digidol (DOIs)	https://doi.org/10.1093/jxb/46.special_issue.1345
Statws	Cyhoeddwyd - 1995

Mynediad at Ddogfen

10.1093/jxb/46.special_issue.1345

Cysylltiad parhaol

Dolen barhaus

Ffeiliau eraill a chysylltiadau

http://hdl.handle.net/2160/43365

Dyfynnu hyn

@article{0d3a8ac63d8d4960b871f6a0f3ae7455,

title = "Isolation of a gene expressed during sucrose accumulation in leaves of Lolium temulentum L.",

abstract = "A 1620 base pair cDNA clone showing increased expression during sucrose accumulation in leaves of Lolium temulentum has been isolated by differential screening. Comparison of the DNA sequence of the open reading frame with published sequences has revealed extensive homology with sequences coding for ATP: L-methionme S-adenosylmethionine synthe-tase, EC 2.5.1.6 (SAM synthetase) (79.6% with Arabidopsis thaliana and 81.6% with Dianthus cary-ophyllus). Increased expression was observed in both intact seedling leaves and detached mature leaves during sucrose accumulation. Expression decreased during sucrose mobilization in leaves of intact seedlings. The significance of increased expression of a gene coding for SAM synthetase during sucrose accumulation is discussed in relation to the involvement of sucrose in the regulation of plant metabolism.",

keywords = "leaf, sucrose, gene expression, S-adenosyimethionine synthetase",

author = "Winters, {A. L.} and J. Gallagher and Pollock, {C. J.} and Parrar, {J. F.}",

year = "1995",

doi = "10.1093/jxb/46.special_issue.1345",

language = "English",

volume = "46",

pages = "1345--1350",

journal = "Journal of Experimental Botany",

issn = "0022-0957",

publisher = "Oxford University Press",

number = "Special Issue",

}

TY - JOUR

T1 - Isolation of a gene expressed during sucrose accumulation in leaves of Lolium temulentum L.

AU - Winters, A. L.

AU - Gallagher, J.

AU - Pollock, C. J.

AU - Parrar, J. F.

PY - 1995

Y1 - 1995

N2 - A 1620 base pair cDNA clone showing increased expression during sucrose accumulation in leaves of Lolium temulentum has been isolated by differential screening. Comparison of the DNA sequence of the open reading frame with published sequences has revealed extensive homology with sequences coding for ATP: L-methionme S-adenosylmethionine synthe-tase, EC 2.5.1.6 (SAM synthetase) (79.6% with Arabidopsis thaliana and 81.6% with Dianthus cary-ophyllus). Increased expression was observed in both intact seedling leaves and detached mature leaves during sucrose accumulation. Expression decreased during sucrose mobilization in leaves of intact seedlings. The significance of increased expression of a gene coding for SAM synthetase during sucrose accumulation is discussed in relation to the involvement of sucrose in the regulation of plant metabolism.

AB - A 1620 base pair cDNA clone showing increased expression during sucrose accumulation in leaves of Lolium temulentum has been isolated by differential screening. Comparison of the DNA sequence of the open reading frame with published sequences has revealed extensive homology with sequences coding for ATP: L-methionme S-adenosylmethionine synthe-tase, EC 2.5.1.6 (SAM synthetase) (79.6% with Arabidopsis thaliana and 81.6% with Dianthus cary-ophyllus). Increased expression was observed in both intact seedling leaves and detached mature leaves during sucrose accumulation. Expression decreased during sucrose mobilization in leaves of intact seedlings. The significance of increased expression of a gene coding for SAM synthetase during sucrose accumulation is discussed in relation to the involvement of sucrose in the regulation of plant metabolism.

KW - leaf

KW - sucrose

KW - gene expression

KW - S-adenosyimethionine synthetase

UR - http://hdl.handle.net/2160/43365

U2 - 10.1093/jxb/46.special_issue.1345

DO - 10.1093/jxb/46.special_issue.1345

M3 - Article

SN - 0022-0957

VL - 46

SP - 1345

EP - 1350

JO - Journal of Experimental Botany

JF - Journal of Experimental Botany

IS - Special Issue

ER -

Isolation of a gene expressed during sucrose accumulation in leaves of Lolium temulentum L.

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Mynediad at Ddogfen

Cysylltiad parhaol

Ffeiliau eraill a chysylltiadau

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