Known Allergen Structures Predict Schistosoma mansoni IgE-Binding Antigens in Human Infection

Edward J Farnell, Nidhi Tyagi, Stephanie Ryan, Iain W Chalmers, Angela Pinot de Moira, Frances M Jones, Jakub Wawrzyniak, Colin M Fitzsimmons, Edridah M. Tukahebwa, Nicholas Furnham, Rick M. Maizels, David W Dunne

Allbwn ymchwil: Cyfraniad at gyfnodolynErthygladolygiad gan gymheiriaid

21 Dyfyniadau(SciVal)
159 Wedi eu Llwytho i Lawr (Pure)

Crynodeb

The IgE response has been associated with both allergic reactions and immunity to metazoan parasites. Recently, we hypothesized that all environmental allergens bear structural homology to IgE-binding antigens from metazoan parasites and that this homology defines the relatively small number of protein families containing allergenic targets. In this study, known allergen structures (Pfam domains) from major environmental allergen families were used to predict allergen-like (SmProfilin, SmVAL-6, SmLipocalin, SmHSP20, Sm triosephosphate isomerase, SmThioredoxin, Sm superoxide dismutase, SmCyclophilin, and Sm phosphoglycerate kinase) and non-allergen-like [Sm dynein light chain (SmDLC), SmAldolase SmAK, SmUbiquitin, and Sm14-3-3] proteins in Schistosoma mansoni. Recombinant antigens were produced in Escherichia coli and IgG1, IgG4, and IgE responses against them measured in a cohort of people (n = 222) infected with S. mansoni. All allergen-like antigens were targeted by IgE responses in infected subjects, whilst IgE responses to the non-allergen-like antigens, SmAK, SmUbiquitin, and Sm14-3-3 were essentially absent being of both low prevalence and magnitude. Two new IgE-binding Pfam domain families, not previously described in allergen family databases, were also found, with prevalent IgE responses against SmDLC (PF01221) and SmAldolase (PF00274). Finally, it was demonstrated that immunoregulatory serological processes typically associated with allergens also occurred in responses to allergen-like proteins in S. mansoni infections, including the production of IgG4 in people responding with IgE and the down-regulation of IgE in response to increased antigen exposure from S. mansoni eggs. This study establishes that structures of known allergens can be used to predict IgE responses against homologous parasite allergen-like molecules (parallergens) and that serological responses with IgE/IgG4 to parallergens mirror those seen against allergens, supporting our hypothesis that allergenicity is rooted in expression of certain protein domain families in metazoan parasites.

Iaith wreiddiolSaesneg
Tudalennau (o-i)26
CyfnodolynFrontiers in Immunology
Cyfrol6
Dynodwyr Gwrthrych Digidol (DOIs)
StatwsCyhoeddwyd - 03 Chwef 2015

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Gweld gwybodaeth am bynciau ymchwil 'Known Allergen Structures Predict Schistosoma mansoni IgE-Binding Antigens in Human Infection'. Gyda’i gilydd, maen nhw’n ffurfio ôl bys unigryw.

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