TY - JOUR
T1 - Live Salmonella enterica serovar Typhimurium (S. Typhimurium) elicit dendritic cell responses that differ from those induced by killed S. Typhimurium
AU - Norimatsu, Mari
AU - Chance, Victoria
AU - Dougan, Gordon
AU - Howard, Christopher J.
AU - Villarreal-Ramos, Bernardo
PY - 2004/4/1
Y1 - 2004/4/1
N2 - The immune response of bovine monocytes-derived dendritic cells (DC) exposed to either live or killed Salmonella enterica serovar Typhimurium was compared. Both live and killed bacteria induced changes in morphology with distinctive formation of processes and up-regulation of the ability of DC to stimulate allogeneic T-cell proliferation. Also, both live and killed bacteria up-regulated the expression of MHC-I, MHC-II and CD80. However, live bacteria induced greater up-regulation of the expression of CD40 and CD86 than killed bacteria. Live bacteria also induced greater up-regulation of transcription for IL-6, IL-12 and GM-CSF than killed bacteria as measured by quantitative RT-PCR. These data suggest that blood-monocyte-derived DC may follow distinct maturation pathways following exposure to live or killed bacteria. These differences are likely to have consequences for the priming of the adaptive immune responses.
AB - The immune response of bovine monocytes-derived dendritic cells (DC) exposed to either live or killed Salmonella enterica serovar Typhimurium was compared. Both live and killed bacteria induced changes in morphology with distinctive formation of processes and up-regulation of the ability of DC to stimulate allogeneic T-cell proliferation. Also, both live and killed bacteria up-regulated the expression of MHC-I, MHC-II and CD80. However, live bacteria induced greater up-regulation of the expression of CD40 and CD86 than killed bacteria. Live bacteria also induced greater up-regulation of transcription for IL-6, IL-12 and GM-CSF than killed bacteria as measured by quantitative RT-PCR. These data suggest that blood-monocyte-derived DC may follow distinct maturation pathways following exposure to live or killed bacteria. These differences are likely to have consequences for the priming of the adaptive immune responses.
KW - Bovine
KW - DC
KW - Dendritic cells
KW - Killed
KW - Live
KW - MΦ
KW - Macrophages
KW - Salmonella Typhimurium
UR - http://www.scopus.com/inward/record.url?scp=1542358762&partnerID=8YFLogxK
U2 - 10.1016/j.vetimm.2003.12.008
DO - 10.1016/j.vetimm.2003.12.008
M3 - Article
C2 - 15010228
AN - SCOPUS:1542358762
SN - 0165-2427
VL - 98
SP - 193
EP - 201
JO - Veterinary Immunology and Immunopathology
JF - Veterinary Immunology and Immunopathology
IS - 3-4
ER -