Previously we have shown that a bovine mammary epithelial cell clone isolated from lactating mammary tissue is able to differentiate morphologically and functionally on a reconstituted basement membrane. Here we show that when cultured on collagen I coated culture inserts, the presence of lactogenic hormones results in an increase in polarized secretion of alpha-lactalbumin but not alpha-casein. We conclude that collagen I is sufficient for milk protein gene expression and that culture insert methodology offers a practical alternative to three-dimensional culture systems when alpha-lactalbumin is used as an indicator of mammary specific function.
|Nifer y tudalennau||4|
|Cyfnodolyn||Journal of Animal and Feed Sciences|
|Rhif cyhoeddi||Supplement 1|
|Statws||Cyhoeddwyd - 2004|
|Digwyddiad||10th International Symposium on Ruminant Pysiology - Copenhagen, Denmarc|
Hyd: 30 Awst 2004 → 04 Medi 2004