Prosiectau fesul blwyddyn
The Pc54 oat line carries the crown rust resistance gene Pc54 and an unknown gene effective against powdery mildew. In this study, two recombinant inbred line (RIL) populations were developed to identify the genomic locations of the two genes and produce lists of molecular markers with a potential for marker-assisted selection. The RILs and parents were phenotyped for crown rust and powdery mildew in a controlled environment. They were also genotyped using the 6K Illumina Infinium iSelect oat single nucleotide polymorphism (SNP) chip. Multiple interval mapping placed Pc54 on the linkage group Mrg02 (chromosome 7D) and the novel powdery mildew quantitative trait locus (QTL) QPm.18 on Mrg18 (chromosome 1A) both in mapping and in the validating populations. A total of 9 and 31 significant molecular markers were identified linked with the Pc54 gene and QPm.18, respectively. Reactions to crown rust inoculations have justified separate identities of Pc54 from other genes and QTLs that have previously been reported on Mrg02 except for qPCRFd. Pm3 is the only powdery mildew resistance gene previously mapped on Mrg18. However, the pm3 differential line, Mostyn, was susceptible to the powdery mildew race used in this study, suggesting that Pm3 and QPm.18 are different genes. Determining the chromosomal locations of Pc54 and QPm.18 is helpful for better understanding of the molecular mechanism of resistance to crown rust and powdery mildew in oats. Furthermore, SNPs and single sequence repeats that are closely linked with the genes could be valuable for developing PCR-based molecular markers and facilitating the utilization of these genes in oat breeding programs.
|Nifer y tudalennau||7|
|Dyddiad ar-lein cynnar||25 Ebr 2022|
|Dynodwyr Gwrthrych Digidol (DOIs)|
|Statws||Cyhoeddwyd - 27 Mai 2022|
Ôl bysGweld gwybodaeth am bynciau ymchwil 'Mapping of Crown Rust (Puccinia coronata f. sp. avenae) Resistance Gene Pc54 and a Novel Quantitative Trait Locus Effective Against Powdery Mildew (Blumeria graminis f. sp. avenae) in the Oat (Avena sativa) Line Pc54'. Gyda’i gilydd, maen nhw’n ffurfio ôl bys unigryw.
- 1 Wedi Gorffen
BBSRC Core Strategic Programme in Resilient Crops: Oats
01 Ebr 2017 → 31 Maw 2022
Prosiect: Ymchwil a ariannwyd yn allanol