Methods for Delivery of dsRNAi Against Canonical Clock Genes and Immunocytodetection of Clock Proteins in Crustacea

David C. Wilcockson, Lin Zhang, Charalambos P. Kyriacou*

*Awdur cyfatebol y gwaith hwn

Allbwn ymchwil: Pennod mewn Llyfr/Adroddiad/Trafodion CynhadleddPennod

1 Dyfyniadau(SciVal)

Crynodeb

The use of nonclassical model organisms for biological rhythm research has become popular in the last two decades. Here we describe techniques for delivery of dsRNAi molecules to knock down clock gene transcripts in a small intertidal crustacean, Eurydice pulchra, as well as our method for immunodetection of clock proteins in the brain. These methods can be generalized for gene knockdown in any small crustacean or arthropod in which mutagenesis by other methods is neither practical nor possible.

Iaith wreiddiolSaesneg
TeitlMethods in Molecular Biology
CyhoeddwrHumana Press Inc.
Tudalennau385-394
Nifer y tudalennau10
ISBN (Electronig)978-1-0716-2249-0
ISBN (Argraffiad)978-1-0716-2248-3
Dynodwyr Gwrthrych Digidol (DOIs)
StatwsCyhoeddwyd - 2022

Cyfres gyhoeddiadau

EnwMethods in Molecular Biology
Cyfrol2482
ISSN (Argraffiad)1064-3745
ISSN (Electronig)1940-6029

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