Allbwn Ymchwil fesul blwyddyn
Allbwn Ymchwil fesul blwyddyn
P. J. Alexander, L. B. Oyama, H. Olleik, F. Godoy Santos, S. O'Brien, A. Cookson, S. A. Cochrane, B. F. Gilmore, M. Maresca, S. A. Huws
Allbwn ymchwil: Cyfraniad at gyfnodolyn › Erthygl › adolygiad gan gymheiriaid
Acinetobacter baumannii is designated by the World Health Organisation as a critical priority pathogen. Previously we discovered antimicrobial peptides (AMPs), namely Lynronne-1, -2 and -3, with efficacy against bacterial pathogens, such as Staphylococcus aureus and Pseudomonas aeruginosa. Here we assessed Lynronne-1, -2 and -3 structure by circular dichroism and efficacy against clinical strains of A. baumannii. All Lynronne AMPs demonstrated alpha-helical secondary structures and had antimicrobial activity towards all tested strains of A. baumannii (Minimum Inhibitory Concentrations 2-128 μg/ml), whilst also having anti-biofilm activity. Lynronne-2 and -3 demonstrated additive effects with amoxicillin and erythromycin, and synergy with gentamicin. The AMPs demonstrated little toxicity towards mammalian cell lines or Galleria mellonella. Fluorescence-based assay data demonstrated that Lynronne-1 and -3 had higher membrane-destabilising action against A. baumannii in comparison with Lynronne-2, which was corroborated by transcriptomic analysis. For the first time, we demonstrate the therapeutic activity of Lynronne AMPs against A. baumannii.
Iaith wreiddiol | Saesneg |
---|---|
Cyfnodolyn | npj Biofilms and Microbiomes |
Cyfrol | 10 |
Rhif cyhoeddi | 1 |
Dynodwyr Gwrthrych Digidol (DOIs) | |
Statws | Cyhoeddwyd - 30 Medi 2024 |
Allbwn ymchwil: Papur gweithio › Rhagargraffiad