Prosiectau fesul blwyddyn
Crynodeb
Gut microbiomes, such as the rumen, greatly influence host nutrition due to their feed energy-harvesting capacity. We investigated temporal ecological interactions facilitating energy harvesting at the fresh perennial ryegrass (PRG)-biofilm interface in the rumen using an in sacco approach and prokaryotic metatranscriptomic profiling.
Results
Network analysis identified two distinct sub-microbiomes primarily representing primary (≤ 4 h) and secondary (≥ 4 h) colonisation phases and the most transcriptionally active bacterial families (i.e Fibrobacteriaceae, Selemondaceae and Methanobacteriaceae) did not interact with either sub-microbiome, indicating non-cooperative behaviour. Conversely, Prevotellaceae had most transcriptional activity within the primary sub-microbiome (focussed on protein metabolism) and Lachnospiraceae within the secondary sub-microbiome (focussed on carbohydrate degradation). Putative keystone taxa, with low transcriptional activity, were identified within both sub-microbiomes, highlighting the important synergistic role of minor bacterial families; however, we hypothesise that they may be ‘cheating’ in order to capitalise on the energy-harvesting capacity of other microbes. In terms of chemical cues underlying transition from primary to secondary colonisation phases, we suggest that AI-2-based quorum sensing plays a role, based on LuxS gene expression data, coupled with changes in PRG chemistry.
Conclusions
In summary, we show that fresh PRG-attached prokaryotes are resilient and adapt quickly to changing niches. This study provides the first major insight into the complex temporal ecological interactions occurring at the plant-biofilm interface within the rumen. The study also provides valuable insights into potential plant breeding strategies for development of the utopian plant, allowing optimal sustainable production of ruminants.
Iaith wreiddiol | Saesneg |
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Rhif yr erthygl | 143 |
Nifer y tudalennau | 17 |
Cyfnodolyn | Microbiome |
Cyfrol | 9 |
Rhif cyhoeddi | 1 |
Dyddiad ar-lein cynnar | 21 Meh 2021 |
Dynodwyr Gwrthrych Digidol (DOIs) | |
Statws | Cyhoeddwyd - 01 Rhag 2021 |
Ôl bys
Gweld gwybodaeth am bynciau ymchwil 'Microbiomes attached to fresh perennial ryegrass are temporally resilient and adapt to changing ecological niches'. Gyda’i gilydd, maen nhw’n ffurfio ôl bys unigryw.Setiau Data
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Plant-attached microbiomes temporally transition into specialised ecological niches
Huws, S., Edwards, J. E., Lin, W., Rubino, F., Alston, M., Swarbreck, D., Caim, S., Rees Stevens, P., Pachebat, J., Won, M.-Y., Oyama, L. B., Creevey, C. & Kingston-Smith, A., Prifysgol Aberystwyth | Aberystwyth University, 03 Ebr 2020
Dangosydd eitem ddigidol (DOI): 10.17605/OSF.IO/RX9H2
Set ddata
Prosiectau
- 1 Wedi Gorffen
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BBSRC Core Strategic Programme in Resilient Crops: Grasslands Gogerddan
Armstead, I. (Prif Ymchwilydd), Donnison, I. (Cyd-ymchwilydd), Jones, H. (Cyd-ymchwilydd), Skot, L. (Cyd-ymchwilydd), Fernandez Fuentes, N. (Cyd-ymchwilydd), Phillips, D. (Prif Ymchwilydd), Kingston-Smith, A. (Cyd-ymchwilydd) & Bosch, M. (Cyd-ymchwilydd)
Biotechnology and Biological Sciences Research Council
01 Ebr 2017 → 31 Maw 2020
Prosiect: Ymchwil a ariannwyd yn allanol