Molecular and histological identification of the crustacean parasite Hematodinium sp (Alveolata, Syndinea) in the shore crab Carcinus maenas

Kristina M. Hamilton; David Morritt; Paul W. Shaw

Molecular and histological identification of the crustacean parasite Hematodinium sp (Alveolata, Syndinea) in the shore crab Carcinus maenas

Kristina M. Hamilton^*, David Morritt, Paul W. Shaw

^*Awdur cyfatebol y gwaith hwn

Adran y Gwyddorau Bywyd

Royal Holloway, University of London

Allbwn ymchwil: Cyfraniad at gyfnodolyn › Erthygl › adolygiad gan gymheiriaid

16 Dyfyniadau (Scopus)

Crynodeb

A syndinian parasite of the genus Hematodinium was detected in tissues and haemolymph of Scottish shore crabs (Carcinus maenas) and Norway lobsters (Nephrops norvegicus) by PCR. Analysis of conserved and variable regions of the ribosomal RNA gene found homology in 18S and 5.8S, and high similarity (> 97%) in ITS1 and ITS2, thus indicating that these parasites belong to the same species. Microscopy of the parasites in the haemal sinuses of Scottish C. maenas revealed morphological similar life-cycle stages to Hematodinium perezi identified in C. maenas in 1931 by Chatton and Poisson, and to Hematodinium sp. detected in N. norvegicus.

Iaith wreiddiol	Saesneg
Tudalennau (o-i)	183-192
Nifer y tudalennau	10
Cyfnodolyn	Acta Protozoologica
Cyfrol	46
Rhif cyhoeddi	3
Statws	Cyhoeddwyd - 2007

Cysylltiad parhaol

Dolen barhaus

Ffeiliau eraill a chysylltiadau

http://hdl.handle.net/2160/8924

Dyfynnu hyn

@article{c429720044684664bf5cefc75a153bc9,

title = "Molecular and histological identification of the crustacean parasite Hematodinium sp (Alveolata, Syndinea) in the shore crab Carcinus maenas",

abstract = "A syndinian parasite of the genus Hematodinium was detected in tissues and haemolymph of Scottish shore crabs (Carcinus maenas) and Norway lobsters (Nephrops norvegicus) by PCR. Analysis of conserved and variable regions of the ribosomal RNA gene found homology in 18S and 5.8S, and high similarity (> 97%) in ITS1 and ITS2, thus indicating that these parasites belong to the same species. Microscopy of the parasites in the haemal sinuses of Scottish C. maenas revealed morphological similar life-cycle stages to Hematodinium perezi identified in C. maenas in 1931 by Chatton and Poisson, and to Hematodinium sp. detected in N. norvegicus.",

keywords = "18S, PEREZI, GENE, morphology, DINOFLAGELLATE INFECTION, crustacean, Carcinus maenas, Syndinea, NORWAY LOBSTER, MORTALITY, Nephrops norvegicus, Hematodinium, ITS region, LOBSTER NEPHROPS-NORVEGICUS, DISEASE, rDNA",

author = "Hamilton, {Kristina M.} and David Morritt and Shaw, {Paul W.}",

year = "2007",

language = "English",

volume = "46",

pages = "183--192",

journal = "Acta Protozoologica",

issn = "0065-1583",

publisher = "Jagiellonian University Press",

number = "3",

}

TY - JOUR

T1 - Molecular and histological identification of the crustacean parasite Hematodinium sp (Alveolata, Syndinea) in the shore crab Carcinus maenas

AU - Hamilton, Kristina M.

AU - Morritt, David

AU - Shaw, Paul W.

PY - 2007

Y1 - 2007

N2 - A syndinian parasite of the genus Hematodinium was detected in tissues and haemolymph of Scottish shore crabs (Carcinus maenas) and Norway lobsters (Nephrops norvegicus) by PCR. Analysis of conserved and variable regions of the ribosomal RNA gene found homology in 18S and 5.8S, and high similarity (> 97%) in ITS1 and ITS2, thus indicating that these parasites belong to the same species. Microscopy of the parasites in the haemal sinuses of Scottish C. maenas revealed morphological similar life-cycle stages to Hematodinium perezi identified in C. maenas in 1931 by Chatton and Poisson, and to Hematodinium sp. detected in N. norvegicus.

AB - A syndinian parasite of the genus Hematodinium was detected in tissues and haemolymph of Scottish shore crabs (Carcinus maenas) and Norway lobsters (Nephrops norvegicus) by PCR. Analysis of conserved and variable regions of the ribosomal RNA gene found homology in 18S and 5.8S, and high similarity (> 97%) in ITS1 and ITS2, thus indicating that these parasites belong to the same species. Microscopy of the parasites in the haemal sinuses of Scottish C. maenas revealed morphological similar life-cycle stages to Hematodinium perezi identified in C. maenas in 1931 by Chatton and Poisson, and to Hematodinium sp. detected in N. norvegicus.

KW - 18S

KW - PEREZI

KW - GENE

KW - morphology

KW - DINOFLAGELLATE INFECTION

KW - crustacean

KW - Carcinus maenas

KW - Syndinea

KW - NORWAY LOBSTER

KW - MORTALITY

KW - Nephrops norvegicus

KW - Hematodinium

KW - ITS region

KW - LOBSTER NEPHROPS-NORVEGICUS

KW - DISEASE

KW - rDNA

UR - http://hdl.handle.net/2160/8924

M3 - Article

SN - 0065-1583

VL - 46

SP - 183

EP - 192

JO - Acta Protozoologica

JF - Acta Protozoologica

IS - 3

ER -

Molecular and histological identification of the crustacean parasite Hematodinium sp (Alveolata, Syndinea) in the shore crab Carcinus maenas

Crynodeb

Cysylltiad parhaol

Ffeiliau eraill a chysylltiadau

Ôl bys

Dyfynnu hyn