@inproceedings{d6765d4d8e284f2c9dfe1aa102c516db,
title = "Molecular breeding and functional genomics for tolerance to abiotic stress",
abstract = "Sustainability is a measure of our ability to produce food with the maximium of efficiency combined with the minimum of damage to the environment. Grasslands represent over 40% of all agricultural land in the European Union, and over 70% in the United Kingdom. Whilst Lolium in Europe is considered to be the ideal source of profitable and safe high quality animal forage, its general poor persistency limits its use to favourable growing areas. Fortunately, genes for abiotic stress resistance are transferred readily from closely related Festuca species by conventional breeding technologies. Introgression mapping allows the assembly of desirable gene combinations and molecular markers to assist with their selection in breeding programmes. Additional new androgenesis techniques have led to novel genotypes rarely observed as outcomes of breeding programmes. Lolium × Festuca hybrids display promiscuous chromosome recombination enabling genes from one species to be transferred readily to homoeologous chromosome regions where they both function normally and remain stable. Despite the close homology between Lolium and Festuca species, repetitive DNA sequences differ sufficiently for their genomes to be distinguished, by genomic in situ hybridisation (GISH). This enables the physical mapping of genes for abiotic stress resistance transferred from Festuca to Lolium. Further chromosome recombination between homoeologous Lolium and Festuca sequences enables Festuca introgressions to be “dissected”, and recombination series created. Knowledge of synteny and gene sequences within model species amongst the Poaceae, combined with the development of sequenced molecular markers, and bacterial artificial chromosomes is enabling the isolation of genes for abiotic stress resistance.",
keywords = "lolium/festuca, drought resistance, winter hardiness, introgression mapping, androgenesis, precision breeding",
author = "Humphreys, {Michael W.} and Janet Humphreys and Donnison, {Iain S.} and King, {Ian P.} and Thomas, {Huw M.} and Marc Ghesquiere and J-L. Durand and Rognli, {O. A.} and Zbigniew Zwierzykowski and M. Rapacz",
note = "Humphreys, M. W., Humphreys, J., Donnison, I. S., King, I. P., Thomas, H. M., Guesquiere, M., Durand, J.-E., Rognli, O.-A., Zwierzykowski, Z., Rapacz, M. (2004). Molecular breeding and functional genomics for tolerance to abiotic stress. Developments in Plant Breeding, Molecular Breeding of Forage and Turf. Proceedings 3rd International Symposium on Molecular Breeding of Forage and Turf, Dallas, Texas and Ardmore, Oklahoma, USA, 18-22 May 2003, Hopkins, A.Wang, Z-Y.Mian, R.Sledge, M.Barker, R. E., Vol 11, pp. 61-80 Key words: Lolium/Festuca - drought resistance - winter hardiness - introgression mapping - androgenesis - precision breeding; Proceedings of the 3rd International Symposium, Molecular Breeding and Forage and Turf ; Conference date: 18-05-2003 Through 22-05-2003",
year = "2004",
language = "English",
isbn = "978-1-4020-1867-1",
series = "Developments in Plant Breeding",
publisher = "Springer Nature",
pages = "61--80",
editor = "Andrew Hopkins and Zeng-YU Wang and Rouf Mian and Mary Sledge and Barker, {Reed E.}",
booktitle = "Molecular Breeding of Forage and Turf",
address = "Switzerland",
}