Molecular characterisation of kappa-5, a major antigenic glycoprotein from Schistosoma mansoni eggs

G. Schramm, J. V. Hamilton, C. I. A. Balog, M. Wuhrer, A. Gronow, S. Beckmann, V. Wippersteg, C. G. Grevelding, T. Goldmann, E. Weber, N. W. Brattig, A. M. Deelder, D. W. Dunne, C. H. Hokke, H. Haas, M. J. Doenhoff

Allbwn ymchwil: Cyfraniad at gyfnodolynErthygladolygiad gan gymheiriaid

53 Dyfyniadau(SciVal)

Crynodeb

The major immunopathological consequences of infection with Schistosoma mansoni, a T helper type 2 response and granuloma formation leading to fibrotic tissue damage, are caused by the egg stage of the parasite. Three antigens of S. mansoni eggs, termed IPSE/alpha-1, omega-1 and kappa-5, have been found to be the primary targets of the egg-directed antibody response of the host. Here, we report on the isolation, cloning and characterisation of kappa-5. Apart from an uncharacterised mRNA sequence in S. japonicum, no significant similarities of kappa-5 to known sequences from other species were found. In contrast to IPSE/alpha-1 and omega-1, which have been found only in eggs, kappa-5 was present in miracidia as well as in eggs at the mRNA and protein levels. In eggs, isoforms of kappa-5 were observed with both three and four fully occupied N-glycosylation sites, while in miracidia only one isoform with four N-glycans could be detected. Interestingly, in Western blots sera from S. mansoni-infected Africans were reactive against kappa-5 with IgE and IgG isotype antibodies, but against IPSE/alpha-1 and omega-1 only with IgG antibodies. The further characterisation of kappa-5 as one of the three major egg antigens should help to better understand the immunology and immunopathology of schistosomiasis.
Iaith wreiddiolSaesneg
Tudalennau (o-i)4-14
Nifer y tudalennau11
CyfnodolynMolecular and Biochemical Parasitology
Cyfrol166
Rhif cyhoeddi1
Dynodwyr Gwrthrych Digidol (DOIs)
StatwsCyhoeddwyd - Gorff 2009

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