Multi-substrate chromosome preparations for high throughput FISH

Robert Hasterok; Joanna Dulawa; Glyn M. Jenkins; J. Michael Leggett; Tim Langdon

doi:10.1186/1472-6750-6-20

Multi-substrate chromosome preparations for high throughput FISH

Robert Hasterok, Joanna Dulawa, Glyn M. Jenkins, J. Michael Leggett, Tim Langdon

Adran y Gwyddorau Bywyd

Institute of Grassland and Environmental Research

Allbwn ymchwil: Cyfraniad at gyfnodolyn › Erthygl › adolygiad gan gymheiriaid

29 Dyfyniadau (Scopus)

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Background: A modification of a standard method of fluorescence in situ hybridisation (FISH) is described, by which a combination of several substrates and probes on single microscope slides enables more accurate comparisons of the distribution and abundance of chromosomal sequences and improves the relatively low throughput of standard FISH methods. Results: The utility and application of multi-colour, multi-substrate FISH is illustrated by the simultaneous physical mapping of retrotransposon sequences to three species of Avena, and single locus BAC (bacterial artificial chromosome) clones and rDNA probes to three species of Brachypodium, demonstrating how this would enable better understanding of complex phylogenetic relationships among some of the species belonging to these two genera. Conclusion: The results show that use of multi-substrate chromosome preparations significantly increases the utility of FISH in comparative analyses of the distribution and abundance of chromosomal sequences in closely related plant species.

Iaith wreiddiol	Saesneg
Cyfnodolyn	BMC Biotechnology
Cyfrol	6
Rhif cyhoeddi	20
Dynodwyr Gwrthrych Digidol (DOIs)	https://doi.org/10.1186/1472-6750-6-20
Statws	Cyhoeddwyd - 20 Maw 2006

Mynediad at Ddogfen

10.1186/1472-6750-6-20

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Dolen barhaus

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@article{1241e8f603064885942b08e45102efe8,

title = "Multi-substrate chromosome preparations for high throughput FISH",

abstract = "Background: A modification of a standard method of fluorescence in situ hybridisation (FISH) is described, by which a combination of several substrates and probes on single microscope slides enables more accurate comparisons of the distribution and abundance of chromosomal sequences and improves the relatively low throughput of standard FISH methods. Results: The utility and application of multi-colour, multi-substrate FISH is illustrated by the simultaneous physical mapping of retrotransposon sequences to three species of Avena, and single locus BAC (bacterial artificial chromosome) clones and rDNA probes to three species of Brachypodium, demonstrating how this would enable better understanding of complex phylogenetic relationships among some of the species belonging to these two genera. Conclusion: The results show that use of multi-substrate chromosome preparations significantly increases the utility of FISH in comparative analyses of the distribution and abundance of chromosomal sequences in closely related plant species.",

author = "Robert Hasterok and Joanna Dulawa and Jenkins, {Glyn M.} and Leggett, {J. Michael} and Tim Langdon",

note = "Hasterok, R., Dulawa, J., Jenkins, G. M., Leggett, J. M., Langdon, T. (2006). Multi-substrate chromosome preparations for high throughput FISH. BMC Biotechnology, 6, (20), 13pp. Sponsorship: BBSRC (ISIS award to R.H. 2003–2004)",

year = "2006",

month = mar,

day = "20",

doi = "10.1186/1472-6750-6-20",

language = "English",

volume = "6",

journal = "BMC Biotechnology",

issn = "1472-6750",

publisher = "Springer Nature",

number = "20",

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TY - JOUR

T1 - Multi-substrate chromosome preparations for high throughput FISH

AU - Hasterok, Robert

AU - Dulawa, Joanna

AU - Jenkins, Glyn M.

AU - Leggett, J. Michael

AU - Langdon, Tim

N1 - Hasterok, R., Dulawa, J., Jenkins, G. M., Leggett, J. M., Langdon, T. (2006). Multi-substrate chromosome preparations for high throughput FISH. BMC Biotechnology, 6, (20), 13pp. Sponsorship: BBSRC (ISIS award to R.H. 2003–2004)

PY - 2006/3/20

Y1 - 2006/3/20

N2 - Background: A modification of a standard method of fluorescence in situ hybridisation (FISH) is described, by which a combination of several substrates and probes on single microscope slides enables more accurate comparisons of the distribution and abundance of chromosomal sequences and improves the relatively low throughput of standard FISH methods. Results: The utility and application of multi-colour, multi-substrate FISH is illustrated by the simultaneous physical mapping of retrotransposon sequences to three species of Avena, and single locus BAC (bacterial artificial chromosome) clones and rDNA probes to three species of Brachypodium, demonstrating how this would enable better understanding of complex phylogenetic relationships among some of the species belonging to these two genera. Conclusion: The results show that use of multi-substrate chromosome preparations significantly increases the utility of FISH in comparative analyses of the distribution and abundance of chromosomal sequences in closely related plant species.

AB - Background: A modification of a standard method of fluorescence in situ hybridisation (FISH) is described, by which a combination of several substrates and probes on single microscope slides enables more accurate comparisons of the distribution and abundance of chromosomal sequences and improves the relatively low throughput of standard FISH methods. Results: The utility and application of multi-colour, multi-substrate FISH is illustrated by the simultaneous physical mapping of retrotransposon sequences to three species of Avena, and single locus BAC (bacterial artificial chromosome) clones and rDNA probes to three species of Brachypodium, demonstrating how this would enable better understanding of complex phylogenetic relationships among some of the species belonging to these two genera. Conclusion: The results show that use of multi-substrate chromosome preparations significantly increases the utility of FISH in comparative analyses of the distribution and abundance of chromosomal sequences in closely related plant species.

U2 - 10.1186/1472-6750-6-20

DO - 10.1186/1472-6750-6-20

M3 - Article

SN - 1472-6750

VL - 6

JO - BMC Biotechnology

JF - BMC Biotechnology

IS - 20

ER -

Multi-substrate chromosome preparations for high throughput FISH

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