Mutagenesis in Lotus japonicus

Lotus Japonicus, A General Introduction: Lotus japonicus as a model system.- Lotus-related species and their agronomic importance.-Drought and saline stress.-Symbiotic Processes: Methods for studying nodule development and function.-A procedure for in vitro nodulation studies.- Arbuscular mycorrhiza.-Cell And Molecular Biology Protocols: Concurrent visualization of gusA and lacZ reporter gene expression.- Embedding root and nodule tissue in plastic (BMM).- RNA isolation using phase extraction and LiCl precipitation.-RNA isolation using CsCl gradients.-96-well DNA isolation method.-Functional Genomics: Genome structural analysis.-Transcriptome analysis using cDNA arrays.-Lotus japonicus expression database.-Procedures for mass spectrometric proteome analysis.-Isolation of peribacteroid membranes for proteome analysis.-Metabolome analysis using GC-MS.-Mutants, Gene-Tagging, And Genetic Analysis: Mutagenesis.-A mutant catalogue of Lotus japonicus.- Tilling.-Ds gene tagging.-Mapping and map-based cloning.-An in silico strategy using comparative sequence analysis.-Information transfer: mapping and cloning in other legumes.-Transformation And Reverse Genetic Analysis: Agrobacterium-mediated in vitro transformation.- Induction of hairy roots for symbiotic gene expression studies.-Transformation-regeneration procedure.-Agrobacterium rhizogenes pRi TL-DNA integration system.-Vectors for reverse genetics and expression analysis.-Primary And Secondary Metabolism: Nitrate assimilation;Influence of nitrogen supply.- Nitrate and ammonium assimilatory enzymes.-Transgenic plants affected in nitrate assimilation.-Secondary metabolite profiling.-Phenolic compounds:extraction and analysis.-Elicitation of isoflavan phytoalexins.-Lotus Japonicus Literature:An update of work published on Lotus japonicus.- Index