TY - JOUR
T1 - Nitric oxide and hydrogen sulfide protect plasma membrane integrity and mitigate chromium-induced methylglyoxal toxicity in maize seedlings
AU - Kharbech, Oussama
AU - Sakouhi, Lamia
AU - Ben Massoud, Marouane
AU - Jose Mur, Luis Alejandro
AU - Corpas, Francisco J.
AU - Djebali, Wahbi
AU - Chaoui, Abdelilah
N1 - Funding Information:
The authors are thankful for the financial support from the Tunisian Ministry of Higher Education , Scientific Research and Technology (LR18ES38). Work at the Institute of Biological, Environmental and Rural Sciences, Aberystwyth University , UK was supported by a visiting fellowship grant to Oussama Kharbech. Research in the laboratory of FJC is supported by ERDF co-financed grants financed by the Ministry of Economy and Competitiveness ( AGL2015-65104-P ) and Junta de Andalucía (group BIO192), Spain.
Funding Information:
The authors are thankful for the financial support from the Tunisian Ministry of Higher Education, Scientific Research and Technology (LR18ES38). Work at the Institute of Biological, Environmental and Rural Sciences, Aberystwyth University, UK was supported by a visiting fellowship grant to Oussama Kharbech. Research in the laboratory of FJC is supported by ERDF co-financed grants financed by the Ministry of Economy and Competitiveness (AGL2015-65104-P) and Junta de Andalucía (group BIO192), Spain.
Publisher Copyright:
© 2020 Elsevier Masson SAS
PY - 2020/12/1
Y1 - 2020/12/1
N2 - The present study aims to analyse the potential crosstalk between nitric oxide (NO) and hydrogen sulfide (H2S) in triggering resilience of maize (Zea mays L.) seedlings to hexavalent chromium (Cr VI). Exogenous application of 500 μM sodium nitroprusside (SNP, as a NO donor) or sodium hydrosulfide (NaHS, as a H2S donor) to 9-day-old maize seedlings, countered a Cr (200 μM) -elicited reduction in embryonic axis biomass. Cr caused cellular membrane injury by enhancing the levels of superoxide and hydroxyl radicals as well as methylglyoxal, and 4-hydroxy-2-nonenal. The application of SNP or NaHS considerably improved the endogenous NO and H2S pool, decreased oxidative stress and lipid peroxidation by suppressing lipoxygenase activity and improving some antioxidant enzymes activities in radicles and epicotyls. Radicles were more affected than epicotyls by Cr-stress with enhanced electrolyte leakage and decreased proton extrusion as indicated by lesser H+-ATPase activity. H2S appeared to mitigate Cr toxicity through up-regulated H+-ATPase and glyoxalase pathways and by maintaining optimal GSH levels as downstream effects of ROS and MG suppression. Hence, H2S-mediated the regeneration of GSH pool is associated with the attenuation of MG toxicity by enhancing S-lactoglutathione and D-lactate production. Taken together, our results indicate complementary roles for H2S and GSH to strengthen membrane integrity against Cr stress in maize seedlings.
AB - The present study aims to analyse the potential crosstalk between nitric oxide (NO) and hydrogen sulfide (H2S) in triggering resilience of maize (Zea mays L.) seedlings to hexavalent chromium (Cr VI). Exogenous application of 500 μM sodium nitroprusside (SNP, as a NO donor) or sodium hydrosulfide (NaHS, as a H2S donor) to 9-day-old maize seedlings, countered a Cr (200 μM) -elicited reduction in embryonic axis biomass. Cr caused cellular membrane injury by enhancing the levels of superoxide and hydroxyl radicals as well as methylglyoxal, and 4-hydroxy-2-nonenal. The application of SNP or NaHS considerably improved the endogenous NO and H2S pool, decreased oxidative stress and lipid peroxidation by suppressing lipoxygenase activity and improving some antioxidant enzymes activities in radicles and epicotyls. Radicles were more affected than epicotyls by Cr-stress with enhanced electrolyte leakage and decreased proton extrusion as indicated by lesser H+-ATPase activity. H2S appeared to mitigate Cr toxicity through up-regulated H+-ATPase and glyoxalase pathways and by maintaining optimal GSH levels as downstream effects of ROS and MG suppression. Hence, H2S-mediated the regeneration of GSH pool is associated with the attenuation of MG toxicity by enhancing S-lactoglutathione and D-lactate production. Taken together, our results indicate complementary roles for H2S and GSH to strengthen membrane integrity against Cr stress in maize seedlings.
KW - Chromium
KW - Glutathione
KW - Hydrogen sulfide
KW - Membrane integrity
KW - Methylglyoxal
KW - Nitric oxide
KW - Chromium/toxicity
KW - Reactive Oxygen Species/metabolism
KW - Seedlings/drug effects
KW - Cell Membrane/drug effects
KW - Proton-Translocating ATPases/metabolism
KW - Glutathione/metabolism
KW - Zea mays/drug effects
KW - Pyruvaldehyde/toxicity
KW - Hydrogen Sulfide/pharmacology
KW - Nitric Oxide/pharmacology
UR - http://www.scopus.com/inward/record.url?scp=85094941276&partnerID=8YFLogxK
U2 - 10.1016/j.plaphy.2020.10.017
DO - 10.1016/j.plaphy.2020.10.017
M3 - Article
C2 - 33152643
AN - SCOPUS:85094941276
SN - 0981-9428
VL - 157
SP - 244
EP - 255
JO - Plant Physiology and Biochemistry
JF - Plant Physiology and Biochemistry
ER -