Non‐targeted metabolite profiling reveals host metabolomic reprogramming during the interaction of black pepper with phytophthora capsici

Divya Kattupalli, Artur Pinski, Sweda Sreekumar, Aswathi Usha, Aiswarya Girija, Manfred Beckmann, Luis Alejandro Jose Mur*, Soniya Eppurathu Vasudevan

*Awdur cyfatebol y gwaith hwn

Allbwn ymchwil: Cyfraniad at gyfnodolynErthygladolygiad gan gymheiriaid

5 Dyfyniadau (Scopus)

Crynodeb

Phytophthora capsici is one of the most destructive pathogens causing quick wilt (foot rot) disease in black pepper (Piper nigrum L.) to which no effective resistance has been defined. To better understand the P. nigrum–P. capsici pathosystem, we employed metabolomic approaches based on flow‐infusion electrospray–high‐resolution mass spectrometry. Changes in the leaf metabolome were assessed in infected and systemic tissues at 24 and 48 hpi. Principal Component Analysis of the derived data indicated that the infected leaves showed a rapid metabolic response by 24 hpi whereas the systemic leaves took 48 hpi to respond to the infection. The major sources of variations between infected leaf and systemic leaf were identified, and enrichment pathway analysis indicated, major shifts in amino acid, tricarboxylic acid cycle, nucleotide and vitamin B6 metabolism upon infection. Moreover, the individual metabolites involved in defensive phytohormone signalling were identified. RT‐qPCR analysis of key salicylate and jasmonate biosynthetic genes indicated a transient reduction of expression at 24 hpi but this increased subsequently. Exogenous application of jasmonate and salicylate reduced P. capsici disease symptoms, but this effect was suppressed with the co‐application of abscisic acid. The results are consistent with abscisic acid reprogramming, sa-licylate and jasmonate defences in infected leaves to facilitate the formation of disease. The augmen-tation of salicylate and jasmonate defences could represent an approach through which quick wilt disease could be controlled in black pepper.

Iaith wreiddiolSaesneg
Rhif yr erthygl11433
CyfnodolynInternational Journal of Molecular Sciences
Cyfrol22
Rhif cyhoeddi21
Dynodwyr Gwrthrych Digidol (DOIs)
StatwsCyhoeddwyd - 22 Hyd 2021

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