TY - JOUR
T1 - Painting the chromosomes of Brachypodium
T2 - Current status and future prospects
AU - Idziak, Dominika Ewa
AU - Betekhtin, Alexander
AU - Wolny, Elzbieta
AU - Lesniewska, Karolina
AU - Wright, Jonathan
AU - Febrer, Melanie
AU - Bevan, Michael W.
AU - Jenkins, Glyn
AU - Hasterok, Robert
N1 - Idziak, D., Betekhtin, A., Wolny, E., Lesniewska, K., Wright, J., Febrer, M., Bevan, M. W., Jenkins, G., Hasterok, R. (2011). Painting the chromosomes of Brachypodium—current status and future prospects. Chromosoma, 120 (5), 469-479.
Open access IMPF: 03.84 RONO: 00
PY - 2011/10
Y1 - 2011/10
N2 - Chromosome painting is one of the most powerful and spectacular tools of modern molecular cytogenetics, enabling complex analyses of nuclear genome structure and evolution. For many years, this technique was restricted to the study of mammalian chromosomes, as it failed to work in plant genomes due mainly to the presence of large amounts of repetitive DNA common to all the chromosomes of the complement. The availability of ordered, chromosome-specific BAC clones of Arabidopsis thaliana containing relatively little repetitive genomic DNA enabled the first chromosome painting in dicotyledonous plants. Here, we show for the first time chromosome painting in three different cytotypes of a monocotyledonous plant—the model grass, Brachypodium distachyon. Possible directions of further detailed studies are proposed, such as the evolution of grass karyotypes, the behaviour of meiotic chromosomes, and the analysis of chromosome distribution at interphase.
AB - Chromosome painting is one of the most powerful and spectacular tools of modern molecular cytogenetics, enabling complex analyses of nuclear genome structure and evolution. For many years, this technique was restricted to the study of mammalian chromosomes, as it failed to work in plant genomes due mainly to the presence of large amounts of repetitive DNA common to all the chromosomes of the complement. The availability of ordered, chromosome-specific BAC clones of Arabidopsis thaliana containing relatively little repetitive genomic DNA enabled the first chromosome painting in dicotyledonous plants. Here, we show for the first time chromosome painting in three different cytotypes of a monocotyledonous plant—the model grass, Brachypodium distachyon. Possible directions of further detailed studies are proposed, such as the evolution of grass karyotypes, the behaviour of meiotic chromosomes, and the analysis of chromosome distribution at interphase.
U2 - 10.1007/s00412-011-0326-9
DO - 10.1007/s00412-011-0326-9
M3 - Article
SN - 0009-5915
VL - 120
SP - 469
EP - 479
JO - Chromosoma
JF - Chromosoma
IS - 5
ER -