Painting the chromosomes of Brachypodium: Current status and future prospects

Dominika Ewa Idziak, Alexander Betekhtin, Elzbieta Wolny, Karolina Lesniewska, Jonathan Wright, Melanie Febrer, Michael W. Bevan, Glyn Jenkins, Robert Hasterok

Allbwn ymchwil: Cyfraniad at gyfnodolynErthygladolygiad gan gymheiriaid

50 Dyfyniadau(SciVal)

Crynodeb

Chromosome painting is one of the most powerful and spectacular tools of modern molecular cytogenetics, enabling complex analyses of nuclear genome structure and evolution. For many years, this technique was restricted to the study of mammalian chromosomes, as it failed to work in plant genomes due mainly to the presence of large amounts of repetitive DNA common to all the chromosomes of the complement. The availability of ordered, chromosome-specific BAC clones of Arabidopsis thaliana containing relatively little repetitive genomic DNA enabled the first chromosome painting in dicotyledonous plants. Here, we show for the first time chromosome painting in three different cytotypes of a monocotyledonous plant—the model grass, Brachypodium distachyon. Possible directions of further detailed studies are proposed, such as the evolution of grass karyotypes, the behaviour of meiotic chromosomes, and the analysis of chromosome distribution at interphase.
Iaith wreiddiolSaesneg
Tudalennau (o-i)469-479
Nifer y tudalennau11
CyfnodolynChromosoma
Cyfrol120
Rhif cyhoeddi5
Dyddiad ar-lein cynnar11 Meh 2011
Dynodwyr Gwrthrych Digidol (DOIs)
StatwsCyhoeddwyd - Hyd 2011

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