Pathogen-derived nitric oxide influences formation of the appressorium infection structure in the phytopathogenic fungus Blumeria graminis

Elena Prats, Timothy L. W. Carver, Luis A. J. Mur

Allbwn ymchwil: Cyfraniad at gyfnodolynErthygladolygiad gan gymheiriaid

55 Dyfyniadau(SciVal)

Crynodeb

Nitric oxide (NO) is an important signal in plant resistance to pathogens. Here we report that NO is also generated by Blumeria graminis f.sp. hordei as a pathogenesis determinant on barley. Infection by B. graminis f.sp. hordei is dependent on appressorium formation in order to penetrate the host. Using fluorescent dye diaminofluorescein-2 diacetate (DAF-2DA) and confocal laser scanning microscopy, transient NO generation was detected within the B. graminis f.sp. hordei appressorium during its maturation. To confirm that NO was indeed being measured, DAF-2DA fluorescence was suppressed using a NO scavenger and a mammalian NO synthase inhibitor. Both chemicals affected the number of appressorial lobes produced by the fungus. These data indicate that NO plays a key role in formation of B. graminis f.sp. hordei appressoria.
Iaith wreiddiolSaesneg
Tudalennau (o-i)476-480
Nifer y tudalennau5
CyfnodolynResearch in Microbiology
Cyfrol159
Rhif cyhoeddi6
Dynodwyr Gwrthrych Digidol (DOIs)
StatwsCyhoeddwyd - 13 Ebr 2008

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