Phenolic compounds extraction and analysis

Phenolic compounds make up one of the major families of secondary metabolites in plants, and they represent a diverse group of compounds. Phenolics can be broadly divided into non-soluble compounds such as condensed tannins, lignins, and cell-wall bound hydroxycinammic acids, and soluble phenolics such as phenolic acids, phenylpropanoids, flavonoids and quinones. In this article, a method for extracting and analysing the soluble fraction of these phenolics, using HPLC coupled to photodiode array (PDA) detector, is described along with its uses to profile the flavonoid content of Lotus japonicus.literature available on this plant.