Polyclonal Activation of Murine B Lymphocytes in vitro by Salmonella typhimurium Porins

H. M. Vordermeier; W. G. Bessler

doi:10.1016/S0171-2985(87)80033-5

Polyclonal Activation of Murine B Lymphocytes in vitro by Salmonella typhimurium Porins

H. M. Vordermeier^*, W. G. Bessler

^*Awdur cyfatebol y gwaith hwn

TB buchol, clefydau a rheolaeth

Allbwn ymchwil: Cyfraniad at gyfnodolyn › Erthygl › adolygiad gan gymheiriaid

19 Dyfyniadau (Scopus)

Crynodeb

Porins were prepared from the outer membrane of Salmonella typhimurium and consisted of three polypeptides with similar molecular ratios around 40 kDa, and isoelectric points around 4.7. They constituted potent polyclonal activators for murine B lymphocytes. The compounds were mitogenic towards splenic lymphocytes of lipopolysaccharide responder Balb/c, non-responder (C3H/Hej), and athymic (Balb/c nu/nu) mouse inbred strains, as measured by ³H-thymidine incorporation into DNA. Furthermore, porin-activated B cells differentiated into immunoglobulin-secreting cells, as was measured by an ELISA test. The porins will be valuable in elucidating the molecular mechanism of lymphocyte activation in bacterial infections.

Iaith wreiddiol	Saesneg
Tudalennau (o-i)	245-251
Nifer y tudalennau	7
Cyfnodolyn	Immunobiology
Cyfrol	175
Rhif cyhoeddi	3
Dynodwyr Gwrthrych Digidol (DOIs)	https://doi.org/10.1016/S0171-2985(87)80033-5
Statws	Cyhoeddwyd - 01 Medi 1987

Mynediad at Ddogfen

10.1016/S0171-2985(87)80033-5

Cysylltiad parhaol

Dolen barhaus

Ffeiliau eraill a chysylltiadau

Link to publication in Scopus

Dyfynnu hyn

@article{c5bce2be92334671bf56c27a89b50d3a,

title = "Polyclonal Activation of Murine B Lymphocytes in vitro by Salmonella typhimurium Porins",

abstract = "Porins were prepared from the outer membrane of Salmonella typhimurium and consisted of three polypeptides with similar molecular ratios around 40 kDa, and isoelectric points around 4.7. They constituted potent polyclonal activators for murine B lymphocytes. The compounds were mitogenic towards splenic lymphocytes of lipopolysaccharide responder Balb/c, non-responder (C3H/Hej), and athymic (Balb/c nu/nu) mouse inbred strains, as measured by 3H-thymidine incorporation into DNA. Furthermore, porin-activated B cells differentiated into immunoglobulin-secreting cells, as was measured by an ELISA test. The porins will be valuable in elucidating the molecular mechanism of lymphocyte activation in bacterial infections.",

keywords = "Con A, concanavalin A, ELISA, enzyme-linked immunosorbent assay, lipopolysaccharide, LPS, SDS, sodium dodecyl sulphate, Lymphocyte Activation, Mice, Inbred Strains, Salmonella typhimurium, Porins, B-Lymphocytes/immunology, Bacterial Outer Membrane Proteins/pharmacology, Animals, Mice, In Vitro Techniques, Mitogens",

author = "Vordermeier, {H. M.} and Bessler, {W. G.}",

year = "1987",

month = sep,

day = "1",

doi = "10.1016/S0171-2985(87)80033-5",

language = "English",

volume = "175",

pages = "245--251",

journal = "Immunobiology",

issn = "0171-2985",

publisher = "Elsevier GmbH",

number = "3",

}

TY - JOUR

T1 - Polyclonal Activation of Murine B Lymphocytes in vitro by Salmonella typhimurium Porins

AU - Vordermeier, H. M.

AU - Bessler, W. G.

PY - 1987/9/1

Y1 - 1987/9/1

N2 - Porins were prepared from the outer membrane of Salmonella typhimurium and consisted of three polypeptides with similar molecular ratios around 40 kDa, and isoelectric points around 4.7. They constituted potent polyclonal activators for murine B lymphocytes. The compounds were mitogenic towards splenic lymphocytes of lipopolysaccharide responder Balb/c, non-responder (C3H/Hej), and athymic (Balb/c nu/nu) mouse inbred strains, as measured by 3H-thymidine incorporation into DNA. Furthermore, porin-activated B cells differentiated into immunoglobulin-secreting cells, as was measured by an ELISA test. The porins will be valuable in elucidating the molecular mechanism of lymphocyte activation in bacterial infections.

AB - Porins were prepared from the outer membrane of Salmonella typhimurium and consisted of three polypeptides with similar molecular ratios around 40 kDa, and isoelectric points around 4.7. They constituted potent polyclonal activators for murine B lymphocytes. The compounds were mitogenic towards splenic lymphocytes of lipopolysaccharide responder Balb/c, non-responder (C3H/Hej), and athymic (Balb/c nu/nu) mouse inbred strains, as measured by 3H-thymidine incorporation into DNA. Furthermore, porin-activated B cells differentiated into immunoglobulin-secreting cells, as was measured by an ELISA test. The porins will be valuable in elucidating the molecular mechanism of lymphocyte activation in bacterial infections.

KW - Con A

KW - concanavalin A

KW - ELISA

KW - enzyme-linked immunosorbent assay

KW - lipopolysaccharide

KW - LPS

KW - SDS

KW - sodium dodecyl sulphate

KW - Lymphocyte Activation

KW - Mice, Inbred Strains

KW - Salmonella typhimurium

KW - Porins

KW - B-Lymphocytes/immunology

KW - Bacterial Outer Membrane Proteins/pharmacology

KW - Animals

KW - Mice

KW - In Vitro Techniques

KW - Mitogens

UR - http://www.scopus.com/inward/record.url?scp=0023232968&partnerID=8YFLogxK

U2 - 10.1016/S0171-2985(87)80033-5

DO - 10.1016/S0171-2985(87)80033-5

M3 - Article

C2 - 2824346

AN - SCOPUS:0023232968

SN - 0171-2985

VL - 175

SP - 245

EP - 251

JO - Immunobiology

JF - Immunobiology

IS - 3

ER -

Polyclonal Activation of Murine B Lymphocytes in vitro by Salmonella typhimurium Porins

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Mynediad at Ddogfen

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Ffeiliau eraill a chysylltiadau

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