Polymorphic nuclear microsatellite loci for studies of brown crab, Cancer pagurus L.

Niall J. Mckeown; Paul W. Shaw

doi:10.1111/j.1471-8286.2007.02033.x

Polymorphic nuclear microsatellite loci for studies of brown crab, Cancer pagurus L.

Niall J. Mckeown^*, Paul W. Shaw

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Allbwn ymchwil: Cyfraniad at gyfnodolyn › Erthygl › adolygiad gan gymheiriaid

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Twelve polymorphic microsatellite DNA loci were isolated from the brown crab, Cancer pagurus L., by construction of microsatellite-enriched genomic libraries. Genotyping of 40 individuals from Norfolk (UK) revealed variable levels of locus polymorphism with an average of 7.75 alleles per locus (range 2-22). The observed and expected heterozygosities per locus ranged from 0.025 to 0.868 and from 0.025 to 0.947, respectively. No evidence of linkage disequilibrium was detected between pairs of loci and genotype proportions at all loci conformed to Hardy-Weinberg equilibrium expectations. The microsatellite loci developed constitute a suite of genetic markers applicable to numerous areas of C. pagurus research.

Iaith wreiddiol	Saesneg
Tudalennau (o-i)	653-655
Nifer y tudalennau	3
Cyfnodolyn	Molecular Ecology Resources
Cyfrol	8
Rhif cyhoeddi	3
Dynodwyr Gwrthrych Digidol (DOIs)	https://doi.org/10.1111/j.1471-8286.2007.02033.x
Statws	Cyhoeddwyd - Mai 2008

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10.1111/j.1471-8286.2007.02033.x

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http://hdl.handle.net/2160/8918

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title = "Polymorphic nuclear microsatellite loci for studies of brown crab, Cancer pagurus L.",

abstract = "Twelve polymorphic microsatellite DNA loci were isolated from the brown crab, Cancer pagurus L., by construction of microsatellite-enriched genomic libraries. Genotyping of 40 individuals from Norfolk (UK) revealed variable levels of locus polymorphism with an average of 7.75 alleles per locus (range 2-22). The observed and expected heterozygosities per locus ranged from 0.025 to 0.868 and from 0.025 to 0.947, respectively. No evidence of linkage disequilibrium was detected between pairs of loci and genotype proportions at all loci conformed to Hardy-Weinberg equilibrium expectations. The microsatellite loci developed constitute a suite of genetic markers applicable to numerous areas of C. pagurus research.",

keywords = "paternity, population structure, stock, Crustacea, brachyura, edible crab",

author = "Mckeown, {Niall J.} and Shaw, {Paul W.}",

year = "2008",

month = may,

doi = "10.1111/j.1471-8286.2007.02033.x",

language = "English",

volume = "8",

pages = "653--655",

journal = "Molecular Ecology Resources",

issn = "1755-098X",

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T1 - Polymorphic nuclear microsatellite loci for studies of brown crab, Cancer pagurus L.

AU - Mckeown, Niall J.

AU - Shaw, Paul W.

PY - 2008/5

Y1 - 2008/5

N2 - Twelve polymorphic microsatellite DNA loci were isolated from the brown crab, Cancer pagurus L., by construction of microsatellite-enriched genomic libraries. Genotyping of 40 individuals from Norfolk (UK) revealed variable levels of locus polymorphism with an average of 7.75 alleles per locus (range 2-22). The observed and expected heterozygosities per locus ranged from 0.025 to 0.868 and from 0.025 to 0.947, respectively. No evidence of linkage disequilibrium was detected between pairs of loci and genotype proportions at all loci conformed to Hardy-Weinberg equilibrium expectations. The microsatellite loci developed constitute a suite of genetic markers applicable to numerous areas of C. pagurus research.

AB - Twelve polymorphic microsatellite DNA loci were isolated from the brown crab, Cancer pagurus L., by construction of microsatellite-enriched genomic libraries. Genotyping of 40 individuals from Norfolk (UK) revealed variable levels of locus polymorphism with an average of 7.75 alleles per locus (range 2-22). The observed and expected heterozygosities per locus ranged from 0.025 to 0.868 and from 0.025 to 0.947, respectively. No evidence of linkage disequilibrium was detected between pairs of loci and genotype proportions at all loci conformed to Hardy-Weinberg equilibrium expectations. The microsatellite loci developed constitute a suite of genetic markers applicable to numerous areas of C. pagurus research.

KW - paternity

KW - population structure

KW - stock

KW - Crustacea

KW - brachyura

KW - edible crab

UR - http://hdl.handle.net/2160/8918

U2 - 10.1111/j.1471-8286.2007.02033.x

DO - 10.1111/j.1471-8286.2007.02033.x

M3 - Article

C2 - 21585861

SN - 1755-098X

VL - 8

SP - 653

EP - 655

JO - Molecular Ecology Resources

JF - Molecular Ecology Resources

IS - 3

ER -

Polymorphic nuclear microsatellite loci for studies of brown crab, Cancer pagurus L.

Crynodeb

NDC y CU

Mynediad at Ddogfen

Cysylltiad parhaol

Ffeiliau eraill a chysylltiadau

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