Potential for rapid antibody detection to identify tuberculous cattle with non-reactive tuberculin skin test results

W. Ray Waters, H. Martin Vordermeier, Shelley Rhodes, Bhagwati Khatri, Mitchell V. Palmer, Mayara F. Maggioli, Tyler C. Thacker, Jeffrey T. Nelson, Bruce V. Thomsen, Suelee Robbe-Austerman, Doris M. Bravo Garcia, Mark A. Schoenbaum, Mark S. Camacho, Jean S. Ray, Javan Esfandiari, Paul Lambotte, Rena Greenwald, Adrian Grandison, Alina Sikar-Gang, Konstantin P. Lyashchenko*

*Awdur cyfatebol y gwaith hwn

Allbwn ymchwil: Cyfraniad at gyfnodolynErthygladolygiad gan gymheiriaid

31 Dyfyniadau (Scopus)
64 Wedi eu Llwytho i Lawr (Pure)

Crynodeb

Background: Bovine tuberculosis (TB) control programs generally rely on the tuberculin skin test (TST) for ante-mortem detection of Mycobacterium bovis-infected cattle. Results: Present findings demonstrate that a rapid antibody test based on Dual-Path Platform (DPP®) technology, when applied 1-3 weeks after TST, detected 9 of 11 and 34 of 52 TST non-reactive yet M. bovis-infected cattle from the US and GB, respectively. The specificity of the assay ranged from 98.9% (n = 92, US) to 96.0% (n = 50, GB) with samples from TB-free herds. Multi-antigen print immunoassay (MAPIA) revealed the presence of antibodies to multiple antigens of M. bovis in sera from TST non-reactors diagnosed with TB. Conclusions: Thus, use of serologic assays in series with TST can identify a significant number of TST non-reactive tuberculous cattle for more efficient removal from TB-affected herds.

Iaith wreiddiolSaesneg
Rhif yr erthygl164
Nifer y tudalennau7
CyfnodolynBMC Veterinary Research
Cyfrol13
Rhif cyhoeddi1
Dynodwyr Gwrthrych Digidol (DOIs)
StatwsCyhoeddwyd - 07 Meh 2017

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