Presence and transcriptional activity of anaerobic fungi and agricultural biogas plants

Veronika Dollhofer; Tony M. Callaghan; Gareth Griffith; Michael Lebuhn; Johann Bauer

doi:10.1016/j.biortech.2017.03.116

Presence and transcriptional activity of anaerobic fungi and agricultural biogas plants

Veronika Dollhofer, Tony M. Callaghan, Gareth Griffith, Michael Lebuhn, Johann Bauer

Adran y Gwyddorau Bywyd

Allbwn ymchwil: Cyfraniad at gyfnodolyn › Erthygl › adolygiad gan gymheiriaid

33 Dyfyniadau (Scopus)

368 Wedi eu Llwytho i Lawr (Pure)

Crynodeb

Bioaugmentation with anaerobic fungi (AF2) is promising for improved biogas generation from lignocelluloses-rich substrates. However, before implementing AF into biogas processes it is necessary to investigate their natural occurrence, community structure and transcriptional activity in agricultural biogas plants. Thus, AF were detected with three specific PCR based methods: (i) Copies of their 18S genes were found in 7 of 10 biogas plants. (ii) transcripts of a GH5 endoglucanase gene were present at low level in two digesters, indicating transcriptional cellulolytic activity of AF. (iii) Phylogeny of the AF-community was inferred with the 28S gene. A new Piromyces species was isolated from a PCR-positive digester.

Evidence for AF was only found in biogas plants operated with high proportions of animal feces. Thus, AF were most likely transferred into digesters with animal derived substrates. Additionally, high process temperatures in combination with long retention times seemed to impede AF survival and activity

Iaith wreiddiol	Saesneg
Tudalennau (o-i)	131-139
Nifer y tudalennau	9
Cyfnodolyn	Bioresource Technology
Cyfrol	235
Dyddiad ar-lein cynnar	22 Maw 2017
Dynodwyr Gwrthrych Digidol (DOIs)	https://doi.org/10.1016/j.biortech.2017.03.116
Statws	Cyhoeddwyd - 01 Gorff 2017

Mynediad at Ddogfen

10.1016/j.biortech.2017.03.116

Presence and transcriptional activity of anaerobic fungi in agricultural biogas plantsLlawysgrif awdur wedi’i dderbyn, 1.32 MBTrwydded: CC BY-NC-ND

Cysylltiad parhaol

Dolen barhaus

Ffeiliau eraill a chysylltiadau

http://hdl.handle.net/2160/44852

Gareth Griffith
- gwgaber.acuk
- Adran y Gwyddorau Bywyd - Professor
Unigolyn: Dysgu ac Ymchwil

Dyfynnu hyn

@article{0d7f368509d448368b25b6d0f0691232,

title = "Presence and transcriptional activity of anaerobic fungi and agricultural biogas plants",

abstract = "Bioaugmentation with anaerobic fungi (AF2) is promising for improved biogas generation from lignocelluloses-rich substrates. However, before implementing AF into biogas processes it is necessary to investigate their natural occurrence, community structure and transcriptional activity in agricultural biogas plants. Thus, AF were detected with three specific PCR based methods: (i) Copies of their 18S genes were found in 7 of 10 biogas plants. (ii) transcripts of a GH5 endoglucanase gene were present at low level in two digesters, indicating transcriptional cellulolytic activity of AF. (iii) Phylogeny of the AF-community was inferred with the 28S gene. A new Piromyces species was isolated from a PCR-positive digester.Evidence for AF was only found in biogas plants operated with high proportions of animal feces. Thus, AF were most likely transferred into digesters with animal derived substrates. Additionally, high process temperatures in combination with long retention times seemed to impede AF survival and activity",

keywords = "anaerobic fungi, biogas, lignocellulose utilization, transcriptional activity, strain isolation",

author = "Veronika Dollhofer and Callaghan, {Tony M.} and Gareth Griffith and Michael Lebuhn and Johann Bauer",

note = "This is the author accepted manuscript. The final version is available from Elsevier via http://dx.doi.org/10.1016/j.biortech.2017.03.116",

year = "2017",

month = jul,

day = "1",

doi = "10.1016/j.biortech.2017.03.116",

language = "English",

volume = "235",

pages = "131--139",

journal = "Bioresource Technology",

issn = "0960-8524",

publisher = "Elsevier",

}

TY - JOUR

T1 - Presence and transcriptional activity of anaerobic fungi and agricultural biogas plants

AU - Dollhofer, Veronika

AU - Callaghan, Tony M.

AU - Griffith, Gareth

AU - Lebuhn, Michael

AU - Bauer, Johann

N1 - This is the author accepted manuscript. The final version is available from Elsevier via http://dx.doi.org/10.1016/j.biortech.2017.03.116

PY - 2017/7/1

Y1 - 2017/7/1

N2 - Bioaugmentation with anaerobic fungi (AF2) is promising for improved biogas generation from lignocelluloses-rich substrates. However, before implementing AF into biogas processes it is necessary to investigate their natural occurrence, community structure and transcriptional activity in agricultural biogas plants. Thus, AF were detected with three specific PCR based methods: (i) Copies of their 18S genes were found in 7 of 10 biogas plants. (ii) transcripts of a GH5 endoglucanase gene were present at low level in two digesters, indicating transcriptional cellulolytic activity of AF. (iii) Phylogeny of the AF-community was inferred with the 28S gene. A new Piromyces species was isolated from a PCR-positive digester.Evidence for AF was only found in biogas plants operated with high proportions of animal feces. Thus, AF were most likely transferred into digesters with animal derived substrates. Additionally, high process temperatures in combination with long retention times seemed to impede AF survival and activity

AB - Bioaugmentation with anaerobic fungi (AF2) is promising for improved biogas generation from lignocelluloses-rich substrates. However, before implementing AF into biogas processes it is necessary to investigate their natural occurrence, community structure and transcriptional activity in agricultural biogas plants. Thus, AF were detected with three specific PCR based methods: (i) Copies of their 18S genes were found in 7 of 10 biogas plants. (ii) transcripts of a GH5 endoglucanase gene were present at low level in two digesters, indicating transcriptional cellulolytic activity of AF. (iii) Phylogeny of the AF-community was inferred with the 28S gene. A new Piromyces species was isolated from a PCR-positive digester.Evidence for AF was only found in biogas plants operated with high proportions of animal feces. Thus, AF were most likely transferred into digesters with animal derived substrates. Additionally, high process temperatures in combination with long retention times seemed to impede AF survival and activity

KW - anaerobic fungi

KW - biogas

KW - lignocellulose utilization

KW - transcriptional activity

KW - strain isolation

UR - http://hdl.handle.net/2160/44852

U2 - 10.1016/j.biortech.2017.03.116

DO - 10.1016/j.biortech.2017.03.116

M3 - Article

C2 - 28365340

SN - 0960-8524

VL - 235

SP - 131

EP - 139

JO - Bioresource Technology

JF - Bioresource Technology

ER -

Presence and transcriptional activity of anaerobic fungi and agricultural biogas plants

Crynodeb

Mynediad at Ddogfen

Cysylltiad parhaol

Ffeiliau eraill a chysylltiadau

Ôl bys

Proffiliau

Gareth Griffith

Dyfynnu hyn