Production of an extracellular chitinolytic system byTalaromyces emersonii CBS 814.70.

L. Hendy; J. Gallagher; A. Winters; T. J. Hackett; L. McHale; A. P. McHale

doi:10.1007/BF01088192

Production of an extracellular chitinolytic system byTalaromyces emersonii CBS 814.70.

L. Hendy, J. Gallagher, A. Winters, T. J. Hackett, L. McHale, A. P. McHale

Adran y Gwyddorau Bywyd

Trinity College Dublin

Allbwn ymchwil: Cyfraniad at gyfnodolyn › Erthygl › adolygiad gan gymheiriaid

11 Dyfyniadau (Scopus)

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The thermophilic fungusTalaromyces emersonii CBS 814.70 produces a thermostable extracellular chitinolytic system when cultured on chitin containing media. The chitinolytic system consists of chitinase (EC 3.2.1.14) and N-acetylglucosaminidase (EC 3.2.1.30). Using fluorescent substrate analogues, in zymogram staining of polyacrylamide gradient and isoelectric focusing gels on which the chitinase system was electrophoresed and focused, respectively, it was found that a number of bands could be resolved. Using isoelectric focusing it was observed that at least 4 extracellular forms of chitinase activity are produced.

Iaith wreiddiol	Saesneg
Tudalennau (o-i)	673-678
Nifer y tudalennau	6
Cyfnodolyn	Biotechnology Letters
Cyfrol	12
Rhif cyhoeddi	9
Dynodwyr Gwrthrych Digidol (DOIs)	https://doi.org/10.1007/BF01088192
Statws	Cyhoeddwyd - Medi 1990

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10.1007/BF01088192

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@article{8362903da20541b5974abe11bdcfc470,

title = "Production of an extracellular chitinolytic system byTalaromyces emersonii CBS 814.70.",

abstract = "The thermophilic fungusTalaromyces emersonii CBS 814.70 produces a thermostable extracellular chitinolytic system when cultured on chitin containing media. The chitinolytic system consists of chitinase (EC 3.2.1.14) and N-acetylglucosaminidase (EC 3.2.1.30). Using fluorescent substrate analogues, in zymogram staining of polyacrylamide gradient and isoelectric focusing gels on which the chitinase system was electrophoresed and focused, respectively, it was found that a number of bands could be resolved. Using isoelectric focusing it was observed that at least 4 extracellular forms of chitinase activity are produced.",

author = "L. Hendy and J. Gallagher and A. Winters and Hackett, {T. J.} and L. McHale and McHale, {A. P.}",

year = "1990",

month = sep,

doi = "10.1007/BF01088192",

language = "English",

volume = "12",

pages = "673--678",

journal = "Biotechnology Letters",

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T1 - Production of an extracellular chitinolytic system byTalaromyces emersonii CBS 814.70.

AU - Hendy, L.

AU - Gallagher, J.

AU - Winters, A.

AU - Hackett, T. J.

AU - McHale, L.

AU - McHale, A. P.

PY - 1990/9

Y1 - 1990/9

N2 - The thermophilic fungusTalaromyces emersonii CBS 814.70 produces a thermostable extracellular chitinolytic system when cultured on chitin containing media. The chitinolytic system consists of chitinase (EC 3.2.1.14) and N-acetylglucosaminidase (EC 3.2.1.30). Using fluorescent substrate analogues, in zymogram staining of polyacrylamide gradient and isoelectric focusing gels on which the chitinase system was electrophoresed and focused, respectively, it was found that a number of bands could be resolved. Using isoelectric focusing it was observed that at least 4 extracellular forms of chitinase activity are produced.

AB - The thermophilic fungusTalaromyces emersonii CBS 814.70 produces a thermostable extracellular chitinolytic system when cultured on chitin containing media. The chitinolytic system consists of chitinase (EC 3.2.1.14) and N-acetylglucosaminidase (EC 3.2.1.30). Using fluorescent substrate analogues, in zymogram staining of polyacrylamide gradient and isoelectric focusing gels on which the chitinase system was electrophoresed and focused, respectively, it was found that a number of bands could be resolved. Using isoelectric focusing it was observed that at least 4 extracellular forms of chitinase activity are produced.

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U2 - 10.1007/BF01088192

DO - 10.1007/BF01088192

M3 - Article

SN - 0141-5492

VL - 12

SP - 673

EP - 678

JO - Biotechnology Letters

JF - Biotechnology Letters

IS - 9

ER -

Production of an extracellular chitinolytic system byTalaromyces emersonii CBS 814.70.

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