Production of interleukin-12 as a self-processing 2A polypeptide

Paul J. Chaplin*, Evelyn B. Camon, Barnardo Villarreal-Ramos, Micheal Flint, Martin D. Ryan, Robert A. Collins

*Awdur cyfatebol y gwaith hwn

Allbwn ymchwil: Cyfraniad at gyfnodolynErthygladolygiad gan gymheiriaid

39 Dyfyniadau (Scopus)

Crynodeb

Interleukin-12 (IL.12) is a heterodimeric cytokine composed of two disulfide-linked subunits (p40 and p35) encoded by separate genes. We used the apparent autocleavage property of a 2A peptide from the foot-and-mouth disease virus (FMDV) to express bovine (Bo) IL-12 as a self-processing polypeptide (p402Ap35). We demonstrate that 2A will mediate the cleavage of p402Ap35 into two separate subunits in a manner similar to that observed during the processing of the FMDV polypeptide. Furthermore, this 2A polypeptide encoded a functional heterodimer, which elicited activities associated with IL-12 in other species. We propose that this strategy of self-processing polypeptides may be used in many applications where the coordinated and stoichiometric expression of complex proteins is required.

Iaith wreiddiolSaesneg
Tudalennau (o-i)235-241
Nifer y tudalennau7
CyfnodolynJournal of Interferon and Cytokine Research
Cyfrol19
Rhif cyhoeddi3
Dynodwyr Gwrthrych Digidol (DOIs)
StatwsCyhoeddwyd - 19 Ebr 1999

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