@article{9421275ecd0449e499fcb42e892cf8d4,
title = "Pulmonary inflammation promoted by type-2 dendritic cells is a feature of human and murine schistosomiasis",
abstract = "Schistosomiasis is a parasitic disease affecting over 200 million people in multiple organs, including the lungs. Despite this, there is little understanding of pulmonary immune responses during schistosomiasis. Here, we show type-2 dominated lung immune responses in both patent (egg producing) and pre-patent (larval lung migration) murine Schistosoma mansoni (S. mansoni) infection. Human pre-patent S. mansoni infection pulmonary (sputum) samples revealed a mixed type-1/type-2 inflammatory cytokine profile, whilst a case-control study showed no significant pulmonary cytokine changes in endemic patent infection. However, schistosomiasis induced expansion of pulmonary type-2 conventional dendritic cells (cDC2s) in human and murine hosts, at both infection stages. Further, cDC2s were required for type-2 pulmonary inflammation in murine pre-patent or patent infection. These data elevate our fundamental understanding of pulmonary immune responses during schistosomiasis, which may be important for future vaccine design, as well as for understanding links between schistosomiasis and other lung diseases.",
keywords = "Animals, Case-Control Studies, Cytokines, Dendritic Cells, Humans, Mice, Pneumonia, Schistosoma mansoni/physiology, Schistosomiasis mansoni, Schistosomiasis/parasitology",
author = "Houlder, {E. L.} and Costain, {A. H.} and I. Nambuya and Brown, {S. L.} and Koopman, {J. P. R.} and Langenberg, {M. C. C.} and Janse, {J. J.} and Hoogerwerf, {M. A.} and Ridley, {A. J. L.} and Forde-Thomas, {J. E.} and Colombo, {S. A. P.} and Winkel, {B. M. F.} and Galdon, {A. A.} and Hoffmann, {K. F.} and Cook, {P. C.} and M. Roestenberg and H. Mpairwe and MacDonald, {A. S.}",
note = "Funding Information: The authors thank the participants in the Netherlands and Uganda for taking part in this study. We appreciate the work done by the field and clinical teams at MRC/UVRI and LSHTM Uganda Research Unit, and at LUMC, as well as the guidance of Alison Elliott and Maria Yazdanbakhsh to facilitate this study. We thank members of the Lydia Becker Institute and MacDonald laboratory (University of Manchester) for scientific discussions and some experimental assistance, and the University of Manchester and UVRI and flow cytometry facilities. We thank Matthew Edwards, Semra Kitchen and Alexander Phythian-Adams (GSK) for scientific discussions. William Agace provided the ItgaxcreIrf4flmice. This work was supported by a BBSRC CASE studentship (with GSK) to ELH (BB/P504543/1), MCCIR core, GCRF IAA, HIC-Vac and MRC funding to ASM (MR/W018748/1). K.F.H. and J.F.T. were supported by the Wellcome trust (107475/Z/15/Z). M.R. was supported by a Veni grant (no. 016.156.076) from the Netherlands Organization for Health Research and Development and a Gisela Thier Fellowship (no. 14-0645) from LUMC. Part of the work was conducted at the MRC/UVRI and LSHTM Uganda Research Unit which is jointly funded by the UK Medical Research Council (MRC) part of UK Research and Innovation (UKRI) and the UK Foreign, Commonwealth and Development Office (FCDO) under the MRC/FCDO Concordat agreement and is also part of the EDCTP2 programme supported by the European Union. Funding Information: The authors thank the participants in the Netherlands and Uganda for taking part in this study. We appreciate the work done by the field and clinical teams at MRC/UVRI and LSHTM Uganda Research Unit, and at LUMC, as well as the guidance of Alison Elliott and Maria Yazdanbakhsh to facilitate this study. We thank members of the Lydia Becker Institute and MacDonald laboratory (University of Manchester) for scientific discussions and some experimental assistance, and the University of Manchester and UVRI and flow cytometry facilities. We thank Matthew Edwards, Semra Kitchen and Alexander Phythian-Adams (GSK) for scientific discussions. William Agace provided the ItgaxIrf4 mice. This work was supported by a BBSRC CASE studentship (with GSK) to ELH (BB/P504543/1), MCCIR core, GCRF IAA, HIC-Vac and MRC funding to ASM (MR/W018748/1). K.F.H. and J.F.T. were supported by the Wellcome trust (107475/Z/15/Z). M.R. was supported by a Veni grant (no. 016.156.076) from the Netherlands Organization for Health Research and Development and a Gisela Thier Fellowship (no. 14-0645) from LUMC. Part of the work was conducted at the MRC/UVRI and LSHTM Uganda Research Unit which is jointly funded by the UK Medical Research Council (MRC) part of UK Research and Innovation (UKRI) and the UK Foreign, Commonwealth and Development Office (FCDO) under the MRC/FCDO Concordat agreement and is also part of the EDCTP2 programme supported by the European Union. cre fl Publisher Copyright: {\textcopyright} 2023, The Author(s).",
year = "2023",
month = dec,
day = "1",
doi = "10.1038/s41467-023-37502-z",
language = "English",
volume = "14",
journal = "Nature Communications",
issn = "2041-1723",
publisher = "Springer Nature",
number = "1",
}
