Quantum Cascade Lasers-Based Detection of Nitric Oxide

Gracia Montilla-Bascón, Julien Mandon, Frans J. M. Harren, Luis A. J. Mur, Simona M. Cristescu, Elena Prats

Allbwn ymchwil: Pennod mewn Llyfr/Adroddiad/Trafodion CynhadleddPennod

3 Dyfyniadau(SciVal)

Crynodeb

Despite the established importance of nitric oxide (NO) in many physiological and molecular processes in plants, most methods for quantifying NO are open to criticism This reflects the differing methods either lacking specificity or sensitivity, or even from an undue dependence of results on experimental conditions (i.e., chemical concentrations, pH, etc.). In this chapter we describe a protocol to measure gaseous NO produced by a biological sample using quantum cascade laser (QCL)-based spectroscopy. This technique is based on absorption of the laser light by the NO molecules which have been passed from a biological sample into an optical s cell that is equipped with two mirrors placed at both ends. This design greatly increases the interaction path length with the NO molecules due to multiple reflections of the light coupled inside the cell. Thus, the method is able to provide online, in planta measurements of the dynamics of NO production, being highly selective and sensitive (down to ppbv levels;1 ppbv = part per billion by volume mixing ratio = 1:10-9).

Iaith wreiddiolSaesneg
TeitlNitric Oxide
Is-deitlMethods and Protocols
GolygyddionAlexander Mengel, Christian Lindermayr
CyhoeddwrSpringer Nature
Tudalennau49-57
Nifer y tudalennau9
Cyfrol1747
ISBN (Argraffiad)978-1-4939-7694-2, 149397694X
Dynodwyr Gwrthrych Digidol (DOIs)
StatwsCyhoeddwyd - 12 Mai 2018

Cyfres gyhoeddiadau

EnwMethods in Molecular Biology
CyhoeddwrSpringer Nature
ISSN (Argraffiad)1064-3745

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