Racemases and epimerases operating through a 1,1-proton transfer mechanism: Reactivity, mechanism and inhibition

Matthew D. Lloyd*, Maksims Yevglevskis, Amit Nathubhai, Tony D. James, Michael D. Threadgill, Timothy J. Woodman

*Awdur cyfatebol y gwaith hwn

Allbwn ymchwil: Cyfraniad at gyfnodolynErthygl adolyguadolygiad gan gymheiriaid

83 Wedi eu Llwytho i Lawr (Pure)


Racemases and epimerases catalyse changes in the stereochemical configurations of chiral centres and are of interest as model enzymes and as biotechnological tools. They also occupy pivotal positions within metabolic pathways and, hence, many of them are important drug targets. This review summarises the catalytic mechanisms of PLP-dependent, enolase family and cofactor-independent racemases and epimerases operating by a deprotonation/reprotonation (1,1-proton transfer) mechanism and methods for measuring their catalytic activity. Strategies for inhibiting these enzymes are reviewed, as are specific examples of inhibitors. Rational design of inhibitors based on substrates has been extensively explored but there is considerable scope for development of transition-state mimics and covalent inhibitors and for the identification of inhibitors by high-throughput, fragment and virtual screening approaches. The increasing availability of enzyme structures obtained using X-ray crystallography will facilitate development of inhibitors by rational design and fragment screening, whilst protein models will facilitate development of transition-state mimics.

Iaith wreiddiolSaesneg
Tudalennau (o-i)5952-5984
Nifer y tudalennau33
CyfnodolynChemical Society Reviews
Rhif cyhoeddi10
Dyddiad ar-lein cynnar12 Ebr 2021
Dynodwyr Gwrthrych Digidol (DOIs)
StatwsCyhoeddwyd - 24 Mai 2021

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