RapGene: a fast and accurate strategy for synthetic gene assembly in Escherichia coli

Massimiliano Zampini, Pauline Rees Stevens, Justin Pachebat, Alison Kingston-Smith, Luis Mur, Finbarr Hayes

Allbwn ymchwil: Cyfraniad at gyfnodolynErthygladolygiad gan gymheiriaid

7 Dyfyniadau(SciVal)
160 Wedi eu Llwytho i Lawr (Pure)

Crynodeb

The ability to assemble DNA sequences de novo through efficient and powerful DNA fabrication methods is one of the foundational technologies of synthetic biology. Gene synthesis, in particular, has been considered the main driver for the emergence of this new scientific discipline. Here we describe RapGene, a rapid gene assembly technique which was successfully tested for the synthesis and cloning of both prokaryotic and eukaryotic genes through a ligation independent approach. The method developed in this study is a complete bacterial gene synthesis platform for the quick, accurate and cost effective fabrication and cloning of gene-length sequences that employ the widely used host Escherichia coli.

Iaith wreiddiolSaesneg
Rhif yr erthygl11302
CyfnodolynScientific Reports
Cyfrol5
Dynodwyr Gwrthrych Digidol (DOIs)
StatwsCyhoeddwyd - 11 Meh 2015

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