Review of methodologies and a protocol for the Agrobacterium-mediated transformation of wheat

Huw D Jones; Angela Doherty; Huixia Wu

doi:10.1186/1746-4811-1-5

Review of methodologies and a protocol for the Agrobacterium-mediated transformation of wheat

Huw D Jones, Angela Doherty, Huixia Wu

Adran y Gwyddorau Bywyd

Rothamsted Research

Allbwn ymchwil: Cyfraniad at gyfnodolyn › Erthygl › adolygiad gan gymheiriaid

120 Dyfyniadau (Scopus)

188 Wedi eu Llwytho i Lawr (Pure)

Crynodeb

Since the first report of wheat transformation by Agrobacterium tumefaciens in 1997, various factors that influence T-DNA delivery and regeneration in tissue culture have been further investigated and modified. This paper reviews the current methodology literature describing Agrobacterium transformation of wheat and provides a complete protocol that we have developed and used to produce over one hundred transgenic lines in both spring and winter wheat varieties.

Iaith wreiddiol	Saesneg
Rhif yr erthygl	5
Cyfnodolyn	Plant Methods
Cyfrol	1
Rhif cyhoeddi	1
Dynodwyr Gwrthrych Digidol (DOIs)	https://doi.org/10.1186/1746-4811-1-5
Statws	Cyhoeddwyd - 05 Medi 2005

Mynediad at Ddogfen

10.1186/1746-4811-1-5

Review of methodologies and a protocol for the Agrobacterium-mediated transformation of wheatFersiwn derfynol wedi’i chyhoeddi, 274 KB

Cysylltiad parhaol

Dolen barhaus

Ffeiliau eraill a chysylltiadau

http://hdl.handle.net/2160/42899

Dyfynnu hyn

@article{f8d240d3efc2444194b7910b3d890e7e,

title = "Review of methodologies and a protocol for the Agrobacterium-mediated transformation of wheat",

abstract = "Since the first report of wheat transformation by Agrobacterium tumefaciens in 1997, various factors that influence T-DNA delivery and regeneration in tissue culture have been further investigated and modified. This paper reviews the current methodology literature describing Agrobacterium transformation of wheat and provides a complete protocol that we have developed and used to produce over one hundred transgenic lines in both spring and winter wheat varieties.",

author = "Jones, {Huw D} and Angela Doherty and Huixia Wu",

year = "2005",

month = sep,

day = "5",

doi = "10.1186/1746-4811-1-5",

language = "English",

volume = "1",

journal = "Plant Methods",

issn = "1746-4811",

publisher = "Springer Nature",

number = "1",

}

TY - JOUR

T1 - Review of methodologies and a protocol for the Agrobacterium-mediated transformation of wheat

AU - Jones, Huw D

AU - Doherty, Angela

AU - Wu, Huixia

PY - 2005/9/5

Y1 - 2005/9/5

N2 - Since the first report of wheat transformation by Agrobacterium tumefaciens in 1997, various factors that influence T-DNA delivery and regeneration in tissue culture have been further investigated and modified. This paper reviews the current methodology literature describing Agrobacterium transformation of wheat and provides a complete protocol that we have developed and used to produce over one hundred transgenic lines in both spring and winter wheat varieties.

AB - Since the first report of wheat transformation by Agrobacterium tumefaciens in 1997, various factors that influence T-DNA delivery and regeneration in tissue culture have been further investigated and modified. This paper reviews the current methodology literature describing Agrobacterium transformation of wheat and provides a complete protocol that we have developed and used to produce over one hundred transgenic lines in both spring and winter wheat varieties.

UR - http://hdl.handle.net/2160/42899

U2 - 10.1186/1746-4811-1-5

DO - 10.1186/1746-4811-1-5

M3 - Article

C2 - 16270934

SN - 1746-4811

VL - 1

JO - Plant Methods

JF - Plant Methods

IS - 1

M1 - 5

ER -

Review of methodologies and a protocol for the Agrobacterium-mediated transformation of wheat

Crynodeb

Mynediad at Ddogfen

Cysylltiad parhaol

Ffeiliau eraill a chysylltiadau

Ôl bys

Dyfynnu hyn