TY - JOUR
T1 - Schistosoma mansoni venom allergen-like proteins
T2 - Phylogenetic relationships, stage-specific transcription and tissue localization as predictors of immunological cross-reactivity
AU - Farias, Leonardo P.
AU - Chalmers, Iain W
AU - Perally, Samirah
AU - Rofatto, Henrique K.
AU - Jackson, Colin J.
AU - Brown, Martha
AU - Khouri, Mariana I.
AU - Barbosa, Mayra M. F.
AU - Hensbergen, Paul J.
AU - Hokke, Cornelis H.
AU - Leite, Luciana C. C.
AU - Hoffmann, Karl F
N1 - Funding Information:
This work was supported by grants from the Welcome Trust (UK) ( WT084273/Z/07/Z ) to KFH, Fundação Butantan , Fundação de Amparo à Pesquisa do Estado de São Paulo (Brazil) to LPF and LLC ( 2012/23124-4 ), Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) to LCCL and Coordenação de Aperfeiçoamento de Pessoal de Nível Superior – Brasil (CAPES) – Finance Code 001, and by fellowships from Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP, Brazil) to LPF ( 2008/57946-5 ) and HKF ( 2007/07685-8 ) and from CNPq to MIK ( 160861/2017-9 ). We thank Dra. Eliana Nakano and Ms. Patricia A. Miyasato for supplying the parasite stages and to Alexsander Seixas de Souza for confocal microscopy ( FAPESP 00/11624-5 ) imaging support, all from Instituto Butantan , Brazil.
Funding Information:
This work was supported by grants from the Welcome Trust (UK) (WT084273/Z/07/Z) to KFH, Funda??o Butantan, Funda??o de Amparo ? Pesquisa do Estado de S?o Paulo (Brazil) to LPF and LLC (2012/23124-4), Conselho Nacional de Desenvolvimento Cient?fico e Tecnol?gico (CNPq) to LCCL and Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior ? Brasil (CAPES) ? Finance Code 001, and by fellowships from Funda??o de Amparo ? Pesquisa do Estado de S?o Paulo (FAPESP, Brazil) to LPF (2008/57946-5) and HKF (2007/07685-8) and from CNPq to MIK (160861/2017-9). We thank Dra. Eliana Nakano and Ms. Patricia A. Miyasato for supplying the parasite stages and to Alexsander Seixas de Souza for confocal microscopy (FAPESP 00/11624-5) imaging support, all from Instituto Butantan, Brazil.
Publisher Copyright:
© 2019 The Authors
PY - 2019/7/1
Y1 - 2019/7/1
N2 - Schistosoma mansoni venom allergen-like proteins (SmVALs) are part of a diverse protein superfamily partitioned into two groups (group 1 and group 2). Phylogenetic analyses of group 1 SmVALs revealed that members could be segregated into subclades (A-D); these subclades share similar gene expression patterns across the parasite lifecycle and immunological cross-reactivity. Furthermore, whole-mount in situ hybridization demonstrated that the phylogenetically, transcriptionally and immunologically-related SmVAL4, 10, 18 and 19 (subclade C) were all localized to the pre-acetabular glands of immature cercariae. Our results suggest that SmVAL group 1 phylogenetic relationships, stage-specific transcriptional profiles and tissue localization are predictive of immunological cross-reactivity.
AB - Schistosoma mansoni venom allergen-like proteins (SmVALs) are part of a diverse protein superfamily partitioned into two groups (group 1 and group 2). Phylogenetic analyses of group 1 SmVALs revealed that members could be segregated into subclades (A-D); these subclades share similar gene expression patterns across the parasite lifecycle and immunological cross-reactivity. Furthermore, whole-mount in situ hybridization demonstrated that the phylogenetically, transcriptionally and immunologically-related SmVAL4, 10, 18 and 19 (subclade C) were all localized to the pre-acetabular glands of immature cercariae. Our results suggest that SmVAL group 1 phylogenetic relationships, stage-specific transcriptional profiles and tissue localization are predictive of immunological cross-reactivity.
KW - venom allergen-like
KW - immunological cross reactivity
KW - tissue localisation
KW - schistosoma mansoni
KW - Schistosoma mansoni
KW - Tissue localization
KW - Venom allergen-like
KW - Immunological cross reactivity
KW - Antigens, Helminth/classification
KW - Multigene Family
KW - Dengue Vaccines/immunology
KW - Electrophoresis, Polyacrylamide Gel
KW - Helminth Proteins/classification
KW - Electrophoresis, Gel, Two-Dimensional
KW - Gene Expression Profiling
KW - Phylogeny
KW - Blotting, Western
KW - Cross Reactions
KW - Immune Sera/immunology
KW - Animals
KW - Mass Spectrometry
KW - Schistosoma mansoni/chemistry
KW - Transcription, Genetic
KW - Allergens/classification
UR - http://www.scopus.com/inward/record.url?scp=85066297946&partnerID=8YFLogxK
U2 - 10.1016/j.ijpara.2019.03.003
DO - 10.1016/j.ijpara.2019.03.003
M3 - Article
C2 - 31136745
SN - 0020-7519
VL - 49
SP - 593
EP - 599
JO - International Journal for Parasitology
JF - International Journal for Parasitology
IS - 8
ER -