Sequence heterogeneity of an mpb70 gene analogue in Mycobacterium kansasii

Alison J Woolford; R.glyn Hewinson; Martin Woodward; Jeremy W Dale

doi:10.1111/j.1574-6968.1997.tb10264.x

Sequence heterogeneity of an mpb70 gene analogue in Mycobacterium kansasii

Alison J Woolford, R.glyn Hewinson, Martin Woodward, Jeremy W Dale

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The protein antigen MPB70 is a major component of culture supernatants of Mycobacterium bovis and is an active ingredient of bovine PPD used for skin-testing cattle for tuberculosis. We have shown that Mycobacterium kansasii possesses a similar gene that cross-reacts in a PCR test for M. bovis. Single strand conformational polymorphism analysis, and the DNA sequence of the PCR product, shows differences between M. kansasii strains, supporting the suggestion that M. kansasii is not a homogeneous species.

Iaith wreiddiol	Saesneg
Tudalennau (o-i)	43-48
Nifer y tudalennau	6
Cyfnodolyn	FEMS Microbiology Letters
Cyfrol	148
Rhif cyhoeddi	1
Dynodwyr Gwrthrych Digidol (DOIs)	https://doi.org/10.1111/j.1574-6968.1997.tb10264.x
Statws	Cyhoeddwyd - 01 Maw 1997

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10.1111/j.1574-6968.1997.tb10264.x

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title = "Sequence heterogeneity of an mpb70 gene analogue in Mycobacterium kansasii",

abstract = "The protein antigen MPB70 is a major component of culture supernatants of Mycobacterium bovis and is an active ingredient of bovine PPD used for skin-testing cattle for tuberculosis. We have shown that Mycobacterium kansasii possesses a similar gene that cross-reacts in a PCR test for M. bovis. Single strand conformational polymorphism analysis, and the DNA sequence of the PCR product, shows differences between M. kansasii strains, supporting the suggestion that M. kansasii is not a homogeneous species.",

keywords = "bovine tuberculosis, MPB70 antigen, Mycobacterium kansasii",

author = "Woolford, {Alison J} and R.glyn Hewinson and Martin Woodward and Dale, {Jeremy W}",

year = "1997",

month = mar,

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doi = "10.1111/j.1574-6968.1997.tb10264.x",

language = "English",

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T1 - Sequence heterogeneity of an mpb70 gene analogue in Mycobacterium kansasii

AU - Woolford, Alison J

AU - Hewinson, R.glyn

AU - Woodward, Martin

AU - Dale, Jeremy W

PY - 1997/3/1

Y1 - 1997/3/1

N2 - The protein antigen MPB70 is a major component of culture supernatants of Mycobacterium bovis and is an active ingredient of bovine PPD used for skin-testing cattle for tuberculosis. We have shown that Mycobacterium kansasii possesses a similar gene that cross-reacts in a PCR test for M. bovis. Single strand conformational polymorphism analysis, and the DNA sequence of the PCR product, shows differences between M. kansasii strains, supporting the suggestion that M. kansasii is not a homogeneous species.

AB - The protein antigen MPB70 is a major component of culture supernatants of Mycobacterium bovis and is an active ingredient of bovine PPD used for skin-testing cattle for tuberculosis. We have shown that Mycobacterium kansasii possesses a similar gene that cross-reacts in a PCR test for M. bovis. Single strand conformational polymorphism analysis, and the DNA sequence of the PCR product, shows differences between M. kansasii strains, supporting the suggestion that M. kansasii is not a homogeneous species.

KW - bovine tuberculosis

KW - MPB70 antigen

KW - Mycobacterium kansasii

UR - http://www.scopus.com/inward/record.url?scp=17644440337&partnerID=8YFLogxK

U2 - 10.1111/j.1574-6968.1997.tb10264.x

DO - 10.1111/j.1574-6968.1997.tb10264.x

M3 - Article

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VL - 148

SP - 43

EP - 48

JO - FEMS Microbiology Letters

JF - FEMS Microbiology Letters

IS - 1

ER -

Sequence heterogeneity of an mpb70 gene analogue in Mycobacterium kansasii

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Mynediad at Ddogfen

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