Targeted expression of a ferulic acid esterase from Aspergillus niger in leaves of forage grasses

Marcia M. de O. Buanafina; Tim Langdon; H. Hicks; Barbara D. Hauck; Sue J. Dalton; Phillip Morris

Targeted expression of a ferulic acid esterase from Aspergillus niger in leaves of forage grasses

Marcia M. de O. Buanafina, Tim Langdon, H. Hicks, Barbara D. Hauck, Sue J. Dalton, Phillip Morris

Gwyddorau Bywyd

Allbwn ymchwil: Pennod mewn Llyfr/Adroddiad/Trafodion Cynhadledd › Trafodion Cynhadledd (Nid-Cyfnodolyn fathau)

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Transgenic plants of Festuca arundinacea and Lolium multiflorum were produced expressing a ferulic acid esterase (FAE) gene isolated from Aspergillus niger, following microprojectile bombardment of cell cultures. The level of FAE activity measured from different vectors targeting FAE to the vacuole, ER and apoplast under constitutive promoters showed that the activity was highest in young leaves and increased along the leaf lamina. FAE expression resulted in release of monomeric and dimeric ferulic acids from cell walls on cell death and this was enhanced several fold by the addition of recombinant xylanase. The effect of FAE expression on the monomeric and dimeric cell wall ester linked ferulate content in comparison to control (non-transformed) plants is reported.

Iaith wreiddiol	Saesneg
Teitl	Multifunctional Grassland: Quality Forages, Animal Products and Landscapes
Golygyddion	J- L. Durand, J-C. Emile, C. Huyghe, G. Lemaire
Cyhoeddwr	British Grassland Society
Tudalennau	66-69
Nifer y tudalennau	4
Cyfrol	7
ISBN (Argraffiad)	2950411037
Statws	Cyhoeddwyd - 2002
Digwyddiad	Proceedings of the 19th General Meeting of the European Grassland Federation (EGF) - La Rochelle, Ffrainc Hyd: 27 Mai 2002 → 30 Mai 2002

Cyfres gyhoeddiadau

Enw	Grassland Science in Europe

Cynhadledd

Cynhadledd	Proceedings of the 19th General Meeting of the European Grassland Federation (EGF)
Gwlad/Tiriogaeth	Ffrainc
Dinas	La Rochelle
Cyfnod	27 Mai 2002 → 30 Mai 2002

Mynediad at Ddogfen

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Dyfynnu hyn

Buanafina, M. M. D. O., Langdon, T., Hicks, H., Hauck, B. D., Dalton, S. J., & Morris, P. (2002). Targeted expression of a ferulic acid esterase from Aspergillus niger in leaves of forage grasses. Yn J. L. Durand, J.-C. Emile, C. Huyghe, & G. Lemaire (Gol.), Multifunctional Grassland: Quality Forages, Animal Products and Landscapes (Cyfrol 7, tt. 66-69). (Grassland Science in Europe). British Grassland Society. http://hdl.handle.net/2160/3802

Buanafina, Marcia M. de O. ; Langdon, Tim ; Hicks, H. et al. / Targeted expression of a ferulic acid esterase from Aspergillus niger in leaves of forage grasses. Multifunctional Grassland: Quality Forages, Animal Products and Landscapes. Gol. / J- L. Durand ; J-C. Emile ; C. Huyghe ; G. Lemaire. Cyfrol 7 British Grassland Society, 2002. tt. 66-69 (Grassland Science in Europe).

@inproceedings{7c6a4ed8e28e40e39f71a57661ef0f09,

title = "Targeted expression of a ferulic acid esterase from Aspergillus niger in leaves of forage grasses",

abstract = "Transgenic plants of Festuca arundinacea and Lolium multiflorum were produced expressing a ferulic acid esterase (FAE) gene isolated from Aspergillus niger, following microprojectile bombardment of cell cultures. The level of FAE activity measured from different vectors targeting FAE to the vacuole, ER and apoplast under constitutive promoters showed that the activity was highest in young leaves and increased along the leaf lamina. FAE expression resulted in release of monomeric and dimeric ferulic acids from cell walls on cell death and this was enhanced several fold by the addition of recombinant xylanase. The effect of FAE expression on the monomeric and dimeric cell wall ester linked ferulate content in comparison to control (non-transformed) plants is reported.",

author = "Buanafina, {Marcia M. de O.} and Tim Langdon and H. Hicks and Hauck, {Barbara D.} and Dalton, {Sue J.} and Phillip Morris",

note = "Buanafina, M. M. de O., Langdon, T., Hicks, H., Hauck, B., Dalton, S. J., Morris, P. (2002). Targeted expression of a ferulic acid esterase from Aspergillus niger in leaves of forage grasses. Pages 66-69 in: , Durand, J-L., Emile, J-C., Huyghe, C., Lemaire, G. (Eds). Multifunctional Grassland: Quality Forages, Animal Products and Landscapes. Grassland Science in Europe 7, ISBN:2950411037. Proceedings of the 19th General Meeting of the European Grassland Federation, La Rochelle, France, 27-30 May 2002. RONO: 1700 6729; 1700 3120; Proceedings of the 19th General Meeting of the European Grassland Federation (EGF) ; Conference date: 27-05-2002 Through 30-05-2002",

year = "2002",

language = "English",

isbn = "2950411037",

volume = "7",

series = "Grassland Science in Europe",

publisher = "British Grassland Society",

pages = "66--69",

editor = "Durand, {J- L.} and J-C. Emile and C. Huyghe and G. Lemaire",

booktitle = "Multifunctional Grassland: Quality Forages, Animal Products and Landscapes",

}

Buanafina, MMDO, Langdon, T, Hicks, H, Hauck, BD, Dalton, SJ & Morris, P 2002, Targeted expression of a ferulic acid esterase from Aspergillus niger in leaves of forage grasses. yn JL Durand, J-C Emile, C Huyghe & G Lemaire (gol.), Multifunctional Grassland: Quality Forages, Animal Products and Landscapes. cyfrol. 7, Grassland Science in Europe, British Grassland Society, tt. 66-69, Proceedings of the 19th General Meeting of the European Grassland Federation (EGF), La Rochelle, Ffrainc, 27 Mai 2002. <http://hdl.handle.net/2160/3802>

Targeted expression of a ferulic acid esterase from Aspergillus niger in leaves of forage grasses. / Buanafina, Marcia M. de O.; Langdon, Tim; Hicks, H. et al.
Multifunctional Grassland: Quality Forages, Animal Products and Landscapes. gol. / J- L. Durand; J-C. Emile; C. Huyghe; G. Lemaire. Cyfrol 7 British Grassland Society, 2002. t. 66-69 (Grassland Science in Europe).

Allbwn ymchwil: Pennod mewn Llyfr/Adroddiad/Trafodion Cynhadledd › Trafodion Cynhadledd (Nid-Cyfnodolyn fathau)

TY - GEN

T1 - Targeted expression of a ferulic acid esterase from Aspergillus niger in leaves of forage grasses

AU - Buanafina, Marcia M. de O.

AU - Langdon, Tim

AU - Hicks, H.

AU - Hauck, Barbara D.

AU - Dalton, Sue J.

AU - Morris, Phillip

N1 - Buanafina, M. M. de O., Langdon, T., Hicks, H., Hauck, B., Dalton, S. J., Morris, P. (2002). Targeted expression of a ferulic acid esterase from Aspergillus niger in leaves of forage grasses. Pages 66-69 in: , Durand, J-L., Emile, J-C., Huyghe, C., Lemaire, G. (Eds). Multifunctional Grassland: Quality Forages, Animal Products and Landscapes. Grassland Science in Europe 7, ISBN:2950411037. Proceedings of the 19th General Meeting of the European Grassland Federation, La Rochelle, France, 27-30 May 2002. RONO: 1700 6729; 1700 3120

PY - 2002

Y1 - 2002

N2 - Transgenic plants of Festuca arundinacea and Lolium multiflorum were produced expressing a ferulic acid esterase (FAE) gene isolated from Aspergillus niger, following microprojectile bombardment of cell cultures. The level of FAE activity measured from different vectors targeting FAE to the vacuole, ER and apoplast under constitutive promoters showed that the activity was highest in young leaves and increased along the leaf lamina. FAE expression resulted in release of monomeric and dimeric ferulic acids from cell walls on cell death and this was enhanced several fold by the addition of recombinant xylanase. The effect of FAE expression on the monomeric and dimeric cell wall ester linked ferulate content in comparison to control (non-transformed) plants is reported.

AB - Transgenic plants of Festuca arundinacea and Lolium multiflorum were produced expressing a ferulic acid esterase (FAE) gene isolated from Aspergillus niger, following microprojectile bombardment of cell cultures. The level of FAE activity measured from different vectors targeting FAE to the vacuole, ER and apoplast under constitutive promoters showed that the activity was highest in young leaves and increased along the leaf lamina. FAE expression resulted in release of monomeric and dimeric ferulic acids from cell walls on cell death and this was enhanced several fold by the addition of recombinant xylanase. The effect of FAE expression on the monomeric and dimeric cell wall ester linked ferulate content in comparison to control (non-transformed) plants is reported.

M3 - Conference Proceeding (Non-Journal item)

SN - 2950411037

VL - 7

T3 - Grassland Science in Europe

SP - 66

EP - 69

BT - Multifunctional Grassland: Quality Forages, Animal Products and Landscapes

A2 - Durand, J- L.

A2 - Emile, J-C.

A2 - Huyghe, C.

A2 - Lemaire, G.

PB - British Grassland Society

T2 - Proceedings of the 19th General Meeting of the European Grassland Federation (EGF)

Y2 - 27 May 2002 through 30 May 2002

ER -

Buanafina MMDO, Langdon T, Hicks H, Hauck BD, Dalton SJ, Morris P. Targeted expression of a ferulic acid esterase from Aspergillus niger in leaves of forage grasses. Yn Durand JL, Emile JC, Huyghe C, Lemaire G, golygyddion, Multifunctional Grassland: Quality Forages, Animal Products and Landscapes. Cyfrol 7. British Grassland Society. 2002. t. 66-69. (Grassland Science in Europe).

Targeted expression of a ferulic acid esterase from Aspergillus niger in leaves of forage grasses

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