Transgenic plants of Festuca arundinacea and Lolium multiflorum were produced expressing a ferulic acid esterase (FAE) gene isolated from Aspergillus niger, following microprojectile bombardment of cell cultures. The level of FAE activity measured from different vectors targeting FAE to the vacuole, ER and apoplast under constitutive promoters showed that the activity was highest in young leaves and increased along the leaf lamina. FAE expression resulted in release of monomeric and dimeric ferulic acids from cell walls on cell death and this was enhanced several fold by the addition of recombinant xylanase. The effect of FAE expression on the monomeric and dimeric cell wall ester linked ferulate content in comparison to control (non-transformed) plants is reported.
|Enw||Grassland Science in Europe|
|Cynhadledd||Proceedings of the 19th General Meeting of the European Grassland Federation (EGF)|
|Cyfnod||27 Mai 2002 → 30 Mai 2002|