Research into formal and informal technology transfer between universities and industry in economical developed counties is well-documented. However, such studies are limited in number in developing economies. In the context of developing economies, this study analyses technology transfer offices’ role in university technology transfer to Ghanaian firms. We incorporate informal mechanisms as a moderating variable to explore the role of human interaction in the technology transfer value chain. In a cross-sectional survey in Ghana, using structural equation modelling with 245 firms, our research finds a negative moderating effect of informal mechanisms on the effect of technology transfer offices on innovation performance in firms. The findings are of significance to universities and corporate bodies in economically developing nations such as Ghana. Policies to improve the effect of informal mechanisms of university technology transfer offices are proposed in developing economies.