The cyclin‐dependent kinase G group defines a thermo‐sensitive alternative splicing circuit modulating the expression of Arabidopsis ATU2AF65A

Nicola Cavallari, Candida Nibau, Armin Fuchs, Despoina Dadarou, Andrea Barta, John Doonan

Allbwn ymchwil: Cyfraniad at gyfnodolynErthygladolygiad gan gymheiriaid

39 Dyfyniadau (Scopus)
162 Wedi eu Llwytho i Lawr (Pure)

Crynodeb

The ability to adapt growth and development to temperature variations is crucial to generate plant varieties resilient to predicted temperature changes. However, the mechanisms underlying plant response to progressive increases in temperature have just started to be elucidated. Here, we report that the Cyclin‐dependent Kinase G1 (CDKG1) is a central element in a thermo‐sensitive mRNA splicing cascade that transduces changes in ambient temperature into differential expression of the fundamental spliceosome component, ATU2AF65A. CDKG1 is alternatively spliced in a temperature‐dependent manner. We found that this process is partly dependent on both the Cyclin‐dependent Kinase G2 (CDKG2) and the interacting co‐factor CYCLIN L1 resulting in two distinct messenger RNAs. Relative abundance of both CDKG1 transcripts correlates with ambient temperature and possibly with different expression levels of the associated protein isoforms. Both CDKG1 alternative transcripts are necessary to fully complement the expression of ATU2AF65A across the temperature range. Our data support a previously unidentified temperature‐dependent mechanism based on the alternative splicing of CDKG1 and regulated by CDKG2 and CYCLIN L1. We propose that changes in ambient temperature affect the relative abundance of CDKG1 transcripts and this in turn translates into differential CDKG1 protein expression coordinating the alternative splicing of ATU2AF65A.
Iaith wreiddiolSaesneg
Tudalennau (o-i)1010-1022
Nifer y tudalennau13
CyfnodolynPlant Journal
Cyfrol94
Rhif cyhoeddi6
Dyddiad ar-lein cynnar30 Maw 2018
Dynodwyr Gwrthrych Digidol (DOIs)
StatwsCyhoeddwyd - 30 Meh 2018

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