TY - JOUR
T1 - The effect of enzyme treatment on the in vitro fermentation of lucerne incubated with equine faecal inocula
AU - Longland, Annette C.
AU - Moore-Colyer, Meriel Jean Scott
AU - Murray, Jo-Anne Mary Davina
AU - Dunnett, Catherine
N1 - Murray, J. M. D., Longland, A. C., Moore-Colyer, M. J. S., Dunnett, C. (2005). The effect of enzyme treatment on the in vitro fermentation of lucerne incubated with equine faecal inocula. British Journal of Nutrition, 94, (5), 771-782
Keywords: Enzyme treatment: In vitro fermentation: Equine inocula: Lucerne
Sponsorship: BBSRC
PY - 2005
Y1 - 2005
N2 - A series of experiments was conducted to determine the effects of a fibrolytic enzyme preparation (enzyme 1; E1) on the in vitro fermentation of lucerne incubated with equine faecal inocula. In experiment 1, high-temperature-dried (HT) lucerne was treated with five levels of E1 (0 to 2·4ml/g DM) and incubated at 50°C for 20h. Samples then received a simulated foregut digestion (SFD) treatment before DM and NSP analysis. In experiment 2, HT lucerne was treated with the same enzyme levels used in experiment 1. Samples were then split into two groups; plus or minus an SFD treatment before in vitro fermentation using an equine faecal inoculum. In experiment 3, fresh and wilted lucerne were treated with the same levels of E1 as experiments 1 and 2, incubated at 50°C for 20h, then fermented in vitro. For experiment 4, fresh and wilted lucerne were treated with low levels (0 to 0·008ml/g DM) of E1 before fermentation. E1 significantly (P
AB - A series of experiments was conducted to determine the effects of a fibrolytic enzyme preparation (enzyme 1; E1) on the in vitro fermentation of lucerne incubated with equine faecal inocula. In experiment 1, high-temperature-dried (HT) lucerne was treated with five levels of E1 (0 to 2·4ml/g DM) and incubated at 50°C for 20h. Samples then received a simulated foregut digestion (SFD) treatment before DM and NSP analysis. In experiment 2, HT lucerne was treated with the same enzyme levels used in experiment 1. Samples were then split into two groups; plus or minus an SFD treatment before in vitro fermentation using an equine faecal inoculum. In experiment 3, fresh and wilted lucerne were treated with the same levels of E1 as experiments 1 and 2, incubated at 50°C for 20h, then fermented in vitro. For experiment 4, fresh and wilted lucerne were treated with low levels (0 to 0·008ml/g DM) of E1 before fermentation. E1 significantly (P
U2 - 10.1079/BJN20051561
DO - 10.1079/BJN20051561
M3 - Article
SN - 1475-2662
SP - 771
EP - 782
JO - British Journal of Nutrition
JF - British Journal of Nutrition
ER -