The Yin-Yang of the Green Fluorescent Protein: Impact on Saccharomyces cerevisiae stress resistance

Mélanie Ragon, Lucie Bertheau, Jennifer Dumont, Tiffany Bellanger, Marie Grosselin, Mohini Basu, Eléonore Pourcelot, Walid Horrigue, Emmanuel Denimal, Ambroise Marin, Basile Vaucher, Antoine Berland, Corentin Lepoivre, Sébastien Dupont, Laurent Beney, Hazel Davey, Stéphane Guyot*

*Awdur cyfatebol y gwaith hwn

Allbwn ymchwil: Cyfraniad at gyfnodolynErthygladolygiad gan gymheiriaid

75 Wedi eu Llwytho i Lawr (Pure)


Although fluorescent proteins are widely used as biomarkers (Yin), no study focuses on their influence on the microbial stress response. Here, the Green Fluorescent Protein (GFP) was fused to two proteins of interest in Saccharomyces cerevisiae. Pab1p and Sur7p, respectively involved in stress granules structure and in Can1 membrane domains. These were chosen since questions remain regarding the understanding of the behavior of S. cerevisiae facing different heat kinetics or oxidative stresses. The main results showed that Pab1p-GFP fluorescent mutant displayed a higher resistance than that of the wild type under a heat shock. Moreover, fluorescent mutants exposed to oxidative stresses displayed changes in the cultivability compared to the wild type strain. In silico approaches showed that the presence of the GFP did not influence the structure and so the functionality of the tagged proteins meaning that changes in yeast resistance were certainly related to GFP ROS-scavenging ability (Yang).

Iaith wreiddiolSaesneg
Rhif yr erthygl112603
Nifer y tudalennau12
CyfnodolynJournal of Photochemistry and Photobiology B: Biology
Dyddiad ar-lein cynnar29 Tach 2022
Dynodwyr Gwrthrych Digidol (DOIs)
StatwsCyhoeddwyd - 01 Ion 2023

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