Towards engineered topogenesis of cytochrome b5 and P450 for in vivo transformation of xenobiotics

Naheed N. Kaderbhai, Mustak A. Kaderbhai, Steven Lewis Kelly

Allbwn ymchwil: Pennod mewn Llyfr/Adroddiad/Trafodion CynhadleddTrafodion Cynhadledd (Nid-Cyfnodolyn fathau)

2 Dyfyniadau (Scopus)

Crynodeb

Nature is endowed with catalysts capable of an unprecedented diversity of biotransformations, beyond the capabilities of synthetic chemistries. In a biotechnological context, there is a growing and emerging need to tap this catalytic potential. CYP (cytochrome P450) represents a superfamily of enzymes capable of a diverse array of catalytic activities. Distinct members are engaged in biosynthetic reactions within many organisms, while others have a role in the detoxification of foreign compounds. The latter substrates include medicines, pollutants, pesticides, carcinogens, perfumes and herbicides, representing considerable applied importance for pharmacology and toxicology. CYPs show a high degree of stereo- and regio-specificity for their reactions, which have wide industrial applications. Recombinant CYPs are commonly expressed as active recombinant cytosolic forms in Escherichia coli. However, selective permeability of E. coli to many substrates and products can cause problems with product recovery when using whole-cell systems. To overcome these problems, we have been developing approaches to facilitate export of functional recombinant haemoproteins to the inner membrane, periplasm and the outer membrane of E. coli. Here, we describe the progress in relation to cytochrome b5 and CYPs.
Iaith wreiddiolSaesneg
Teitl8th International Symposium on Cytochrome P450 Biodiversity and Biotechnology
Tudalennau1231-1235
Nifer y tudalennau5
StatwsCyhoeddwyd - 2006
Digwyddiad8th International Symposium on Cytochrome P450 Biodiversity and Biotechnology - Swansea, Teyrnas Unedig Prydain Fawr a Gogledd Iwerddon
Hyd: 23 Gorff 200627 Gorff 2006

Cynhadledd

Cynhadledd8th International Symposium on Cytochrome P450 Biodiversity and Biotechnology
Gwlad/TiriogaethTeyrnas Unedig Prydain Fawr a Gogledd Iwerddon
DinasSwansea
Cyfnod23 Gorff 200627 Gorff 2006

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