Transient transformation of plants

Huw D Jones, Angela Doherty, Caroline A. Sparks

Allbwn ymchwil: Pennod mewn Llyfr/Adroddiad/Trafodion CynhadleddPennod

Crynodeb

Transient expression in plants is a valuable tool for many aspects of functional genomics and promoter testing. It can be used both to over-express and to silence candidate genes. It is also scaleable and provides a viable alternative to microbial fermentation and animal cell culture for the production of recombinant proteins. It does not depend on chromosomal integration of heterologous DNA so is a relatively facile procedure and can lead to high levels of transgene expression. Recombinant DNA can be introduced into plant cells via physical methods, via Agrobacterium or via viral vectors.

Iaith wreiddiolSaesneg
TeitlPlant Genomics
GolygyddionJ. P. Gustafson, Peter Langridge, Daryl J. Somers
CyhoeddwrSpringer Nature
Tudalennau131-152
Nifer y tudalennau22
Cyfrol513
ISBN (Electronig)978-1-59745-427-8
ISBN (Argraffiad)978-1-58829-997-0
Dynodwyr Gwrthrych Digidol (DOIs)
StatwsCyhoeddwyd - 2009

Cyfres gyhoeddiadau

EnwMethods in Molecular Biology
CyhoeddwrHumana Press
Cyfrol513
ISSN (Argraffiad)1064-3745

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