@inbook{6536a27eb40540f3b62fbb3a6f71e891,
title = "Transient transformation of plants",
abstract = "Transient expression in plants is a valuable tool for many aspects of functional genomics and promoter testing. It can be used both to over-express and to silence candidate genes. It is also scaleable and provides a viable alternative to microbial fermentation and animal cell culture for the production of recombinant proteins. It does not depend on chromosomal integration of heterologous DNA so is a relatively facile procedure and can lead to high levels of transgene expression. Recombinant DNA can be introduced into plant cells via physical methods, via Agrobacterium or via viral vectors.",
keywords = "Agrobacterium tumefaciens, Biolistics, DNA, Bacterial, DNA, Recombinant, Gene Expression, Gene Silencing, Genetic Techniques, Genetic Vectors, Plant Viruses, Plants, Genetically Modified, Plasmids, Replicon, Transfection, Transformation, Genetic, Triticum, viral-induced gene silencing, gene delivery",
author = "Jones, {Huw D} and Angela Doherty and Sparks, {Caroline A.}",
year = "2009",
doi = "10.1007/978-1-59745-427-8_8",
language = "English",
isbn = "978-1-58829-997-0",
volume = "513",
series = "Methods in Molecular Biology",
publisher = "Springer Nature",
pages = "131--152",
editor = "Gustafson, {J. P.} and Peter Langridge and Somers, {Daryl J.}",
booktitle = "Plant Genomics",
address = "Switzerland",
}