Untargeted metabolomic analysis of thoracic blood from badgers indicate changes linked to infection with bovine tuberculosis (Mycobacterium bovis): A pilot study

James Scott Bauman, Richard Pizzey, Manfred Beckmann, Bernardo Villarreal-Ramos, Jonathan King, Beverley Hopkins, David Rooke, Glyn Hewinson, Luis A.J. Mur*

*Awdur cyfatebol y gwaith hwn

Allbwn ymchwil: Cyfraniad at gyfnodolynErthygladolygiad gan gymheiriaid

2 Dyfyniadau (Scopus)
101 Wedi eu Llwytho i Lawr (Pure)

Crynodeb

Introduction
Mycobacterium bovis, the causative agent of bovine tuberculosis (bTB) in cattle, represents a major disease burden to UK cattle farming, with considerable costs associated with its control. The European badger (Meles meles) is a known wildlife reservoir for bTB and better knowledge of the epidemiology of bTB through testing wildlife is required for disease control. Current tests available for the diagnosis of bTB in badgers are limited by cost, processing time or sensitivities.

Materials and Methods
We assessed the ability of flow infusion electrospray—high-resolution mass spectrometry (FIE-HRMS) to determine potential differences between infected and non-infected badgers based on thoracic blood samples obtained from badgers found dead in Wales. Thoracic blood samples were autoclaved for handling in a containment level 2 (CL2) hazard laboratory.

Results
Here we show the major differences associated with with M. bovis infection were changes to folate, pyrimidine, histidine, glycerophospholipid and phosphonate metabolism.

Conclusions
Our studies have indicated differences in the metabolomic signature of badgers found dead in relation to their infection status, suggesting metabolomics could hold potential for developing novel diagnostics for bTB in badgers. As well as highlighting a potential way to handle samples containing a highly pathogenic agent at CL2 for metabolomics studies.
Iaith wreiddiolSaesneg
Rhif yr erthygl61
Nifer y tudalennau9
CyfnodolynMetabolomics
Cyfrol18
Rhif cyhoeddi8
Dyddiad ar-lein cynnar27 Gorff 2022
Dynodwyr Gwrthrych Digidol (DOIs)
StatwsCyhoeddwyd - 27 Gorff 2022

Ôl bys

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