Use of earthworm casts to validate FT-IR spectroscopy as a 'sentinel' technology for high-throughput monitoring of global changes in microbial ecology

John Scullion, Geoffrey Nigel Elliott, Wei Huang, Royston Goodacre, Hilary Worgan, Robert M. Darby, Mark J. Bailey, Dylan Gwynn-Jones, Gareth W. Griffith, Michael K. Winson, Peter A. Williams, Christopher D. Clegg, John Draper

Allbwn ymchwil: Cyfraniad at gyfnodolynErthygl

16 Dyfyniadau(SciVal)

Crynodeb

This study aimed to evaluate metabolic fingerprinting by Fourier transform infrared (FT-IR) spectroscopy as a technique for investigating microbial communities and their activities in soil. FT-IR spectra from earthworm casts, and other ‘biosamples’, were compared using multivariate cluster analyses. The work formed part of a wider study to quantify the risk of horizontal gene flow and to assess ecological impacts associated with the release of GM crops or recombinant micro-organisms. A range of samples, including pure cultures of similar soil bacteria, plant materials and earthworm casts of various ages and feeding regimes were analysed. A subset of the cast FT-IR data was compared with DGGE analysis of extracted DNA/RNA. Cluster analysis of FT-IR spectra was capable of differentiating between different bacterial, litter and cast samples. There was congruence between FT-IR and DGGE clustering for food type but not for cast age. Further detailed work on the microbial populations will be needed to investigate relationships between microbial and spectroscopy data.
Iaith wreiddiolSaesneg
Tudalennau (o-i)440-446
Nifer y tudalennau7
CyfnodolynPedobiologia
Cyfrol47
Rhif cyhoeddi5-6
Dynodwyr Gwrthrych Digidol (DOIs)
StatwsCyhoeddwyd - 2003
Digwyddiad7th International Symposium on Earthworm Ecology - Cardiff, Teyrnas Unedig Prydain Fawr a Gogledd Iwerddon
Hyd: 01 Medi 200106 Medi 2002

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