Using next-generation sequencing to determine diversity of horse intestinal worms: Identifying the equine ʼnemabiome'

Catriona Mitchell, Catherine O'Sullivan, Eric Jean-Yves Roger Pinloche, Toby Wilkinson, Russ Morphew, Neil McEwan

Allbwn ymchwil: Cyfraniad at gyfnodolynErthygladolygiad gan gymheiriaid

19 Dyfyniadau (Scopus)
234 Wedi eu Llwytho i Lawr (Pure)

Crynodeb

Next-generation sequencing of DNA from nematode eggs has been utilised to give the first account of the equine ʼnemabiome'. In all equine faecal samples investigated, multiple species of Strongylidae were detected, ranging from 7.5 (SEM 0.79) with 99+% identity to sequences in the NCBI database to 13.3 (SEM 0.80) with 90+% identity. This range is typical of the number of species described previously in morphological studies using large quantities of digesta per animal. However, the current method is non-invasive; relies on DNA analysis, avoiding the need for specialist microscopy identification; and can be carried out with small samples, providing significant advantages over current methods.

Iaith wreiddiolSaesneg
Tudalennau (o-i)1-5
Nifer y tudalennau5
CyfnodolynJournal of Equine Science
Cyfrol30
Rhif cyhoeddi1
Dynodwyr Gwrthrych Digidol (DOIs)
StatwsCyhoeddwyd - Maw 2019

Ôl bys

Gweld gwybodaeth am bynciau ymchwil 'Using next-generation sequencing to determine diversity of horse intestinal worms: Identifying the equine ʼnemabiome''. Gyda’i gilydd, maen nhw’n ffurfio ôl bys unigryw.

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