V67L Mutation Fills an Internal Cavity to Stabilize RecA Mtu Intein

Allison Zwarycz, Martin Fossat, Otar Akanyeti, Zhongqian Lin, David J. Rosenmann, Angel E. Garcia, Catherine A. Royer, Kenneth V. Mills, Chunyu Wang

Allbwn ymchwil: Cyfraniad at gyfnodolynErthygladolygiad gan gymheiriaid

8 Dyfyniadau(SciVal)
62 Wedi eu Llwytho i Lawr (Pure)


Inteins mediate protein splicing, which has found extensive applications in protein science and biotechnology. In the Mycobacterium tuberculosis RecA mini–mini intein (ΔΔIhh), a single valine to leucine substitution at position 67 (V67L) dramatically increases intein stability and activity. However, crystal structures show that the V67L mutation causes minimal structural rearrangements, with a root-mean-square deviation of 0.2 Å between ΔΔIhh-V67 and ΔΔIhh-L67. Thus, the structural mechanisms for V67L stabilization and activation remain poorly understood. In this study, we used intrinsic tryptophan fluorescence, high-pressure nuclear magnetic resonance (NMR), and molecular dynamics (MD) simulations to probe the structural basis of V67L stabilization of the intein fold. Guanidine hydrochloride denaturation monitored by fluorescence yielded free energy changes (ΔGf°) of −4.4 and −6.9 kcal mol–1 for ΔΔIhh-V67 and ΔΔIhh-L67, respectively. High-pressure NMR showed that ΔΔIhh-L67 is more resistant to pressure-induced unfolding than ΔΔIhh-V67 is. The change in the volume of folding (ΔVf) was significantly larger for V67 (71 ± 2 mL mol–1) than for L67 (58 ± 3 mL mol–1) inteins. The measured difference in ΔVf (13 ± 3 mL mol–1) roughly corresponds to the volume of the additional methylene group for Leu, supporting the notion that the V67L mutation fills a nearby cavity to enhance intein stability. In addition, we performed MD simulations to show that V67L decreases side chain dynamics and conformational entropy at the active site. It is plausible that changes in cavities in V67L can also mediate allosteric effects to change active site dynamics and enhance intein activity
Iaith wreiddiolSaesneg
Tudalennau (o-i)2715–2722
Rhif cyhoeddi21
Dyddiad ar-lein cynnar18 Mai 2017
Dynodwyr Gwrthrych Digidol (DOIs)
StatwsCyhoeddwyd - 30 Mai 2017

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