Determining the Most Appropriate Method of Culture of Pig Meat In Vitro

Crynodeb

This thesis aimed to assess methods of isolation and culture of adult porcine satellite cells, with the goal of cultured meat production. Three porcine muscles were compared for the extraction of satellite cells. The extensor carpi radialis proved to yield cells with higher proliferation rates and more robust Pax7+ populations than other muscles studied. Satellite cells were then assessed over the course of five passages. It was shown that a subpopulation of satellite cells maintain their quiescence over multiple passages and do not significantly decrease in proliferation rates, even in adult cells. The effect of cryopreservation upon satellite cells was then tested. The results also suggest that while cryopreservation does have significant effects on the growth rates and satellite cell populations of a culture, a large population of Pax7+cells can survive the effects. Therefore, cryopreservation remains as a potential technique for future use in this field. Finally it was shown that while FGF2 does have some effect on satellite cell proliferation rates, concentrations can be reduced further than previously reported with no significant effect. It was also shown that inclusion of FGF2 in the media composition does not significantly impact on key myogenic protein composition in differentiated cultures. These results in total propose robust and cheap methods of extracting and culturing adult porcine satellite cells in vitro. This thesis suggests methods that can be used in a future cultured pork system

Dyddiad Dyfarnu	2022
Iaith wreiddiol	Saesneg
Sefydliad Dyfarnu	Prifysgol Aberystwyth
Goruchwyliwr	Debbie Nash (Goruchwylydd) & Ruth Wonfor (Goruchwylydd)