Data from: Novel microsatellite markers for epiphytic bromeliad Tillandsia recurvata L., in an urban landscape in South-eastern Brazil

The authors present seven novel microsatellite markers for Tillandsia
recurvata L. The genome assemble sequences of T. recurvata were obtained
from NCBI (7.2Gb) (Sayers et al. 2022). The BioProject Accession and
accession numbers are PRJNA701548 and SRX10089449, respectively. The
microsatellite identification software Krait (0.5.2) (Du et al. 2018) was
used to detect suitable microsatellites, both genome-wide and in the
noncoding regions of T. recurvata. The single sequence repeats (SSRs) were
refined to a minimum number of seven repeats of di-, tri-, or
tetra-nucleotide repeat motifs. These sequences were further limited to
SSRs of more than 100bp in length and low GC content (<50%). Krait
(0.5.2) (Du et al. 2018) was also used to design the primers for the
selected SSR sequences, in conjunction with the integrated Primer3
software. The criteria for primer selection included: a primer length of
18-26bp, an optimal melting temperature (Tm) of 54-59°C and GC content of
<50%. These designed primers were single-plexed and amplified using
the following PCR cycle: initial denaturation (95°C for 3 min), 34 cycles
of 95°C for 30s, annealing for 30s (JP01-JP12: 54°C, 4873TD + 35251TD:
56°C, 19286TD: 53°C, 5044TD + 186664TD + 214633TD: 58°C), 72°C for 1 min
and a final extension of 72°C for 5 mins. The authors make this
information available to other researchers, to continue the investigation
of epiphyte genetics.

The genome assemble sequences of T.
recurvata were obtained from NCBI (7.2Gb) (Sayers et
al. 2022). The BioProject Accession and accession numbers are
PRJNA701548 and SRX10089449, respectively. The microsatellite
identification software Krait (0.5.2) (Du et al.
2018) was used to detect suitable microsatellites, both genome-wide and in
the noncoding regions of T. recurvata. The single
sequence repeats (SSRs) were refined to a minimum number of seven repeats
of di-, tri-, or tetra-nucleotide repeat motifs. These sequences were
further limited to SSRs of more than 100bp in length and low GC content
(<50%). Krait (0.5.2) (Du et al. 2018) was
also used to design the primers for the selected SSR sequences, in
conjunction with the integrated Primer3 software. The criteria for primer
selection included: a primer length of 18-26bp, an optimal melting
temperature (Tm) of 54-59°C and GC content of <50%. These designed
primers were single-plexed and amplified using the following PCR cycle:
initial denaturation (95°C for 3 min), 34 cycles of 95°C for 30s,
annealing for 30s (JP01-JP12: 54°C, 4873TD + 35251TD: 56°C, 19286TD: 53°C,
5044TD + 186664TD + 214633TD: 58°C), 72°C for 1 min and a final extension
of 72°C for 5 mins.

The corresponding author that designed each microsatellite marker
has been indicated in the reference column of the dataset. The authors
invite any correspondence and queries relating to these microsatellite
markers.